Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in Apc (Min/+) mice.
Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in Apc (Min/+) mice.
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DOI：
10.3164/jcbn.13-91
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发表时间：
2014-03
影响因子：
2.4
通讯作者：
Mutoh M
中科院分区：
文献类型：
--
作者：
Shimizu S;Fujii G;Takahashi M;Nakanishi R;Komiya M;Shimura M;Noma N;Onuma W;Terasaki M;Yano T;Mutoh M
间质和上皮细胞中环氧化酶-2分泌过多的前列腺素是结直肠癌发生的一个致病因素。因此，抑制结肠上皮细胞环氧化酶-2转录活性的化合物可能是抗癌药物的候选物。利用β-半乳糖苷酶报告基因系统测定了环氧化酶-2在人结肠癌细胞系DLD-1中的转录活性。利用该系统，我们证明了100µM芝麻酚（芝麻中的一种木脂素）的基础环氧化酶-2转录活性降低了50%。芝麻中的其他化合物，如芝麻素、芝麻素、阿魏酸和丁香酸，在高达100µM的温度下，对环氧化酶-2的转录活性没有明显的抑制作用。在接下来的实验中，6周大的雄性Min小鼠，apc缺陷小鼠，被分为未处理组和500 ppm芝麻酚组。在15周龄时，发现芝麻酚治疗使小肠中部息肉数量减少到未治疗值的66.1%。此外，芝麻酚抑制息肉部位环氧化酶-2和胞浆前列腺素E2合成酶mRNA。本研究结果可能证明了芝麻酚的新型抗癌特性，并暗示抑制环氧化酶-2表达的药物可能是有用的癌症化学预防药物。
Excessive prostaglandin production by cyclooxygenase-2 in stromal and epithelial cells is a causative factor of colorectal carcinogenesis. Thus, compounds which inhibit cyclooxygenase-2 transcriptional activity in colon epithelial cells could be candidates for anti-carcinogenic agents. A cyclooxygenase-2 transcriptional activity in the human colon cancer cell line DLD-1 has been measured using a β-galactosidase reporter gene system. Using this system, we demonstrated that the decrease in basal cyclooxygenase-2 transcriptional activities at 100 µM sesamol, one of the lignans in sesame seeds, was 50%. Other compounds in sesame seeds such as sesamin, sesamolin, ferulic acid, and syringic acid did not exhibit significant suppression of cyclooxygenase-2 transcriptional activity at up to 100 µM. In a following experiment, 6-week-old male Min mice, Apc-deficient mice, were divided into a non-treated and 500 ppm sesamol groups. At the age of 15 weeks, it was found that treatment with sesamol decreased the number of polyps in the middle part of small intestine to 66.1% of the untreated value. Moreover, sesamol suppressed cyclooxygenase-2 and cytosolic prostaglandin E2 synthase mRNA in the polyp parts. The present findings may demonstrate the novel anti-carcinogenetic property of sesamol, and imply that agents that can suppress cyclooxygenase-2 expression may be useful cancer chemopreventive agents.
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影响因子：
82.9
作者：
Sonoshita, M;Takaku, K;Taketo, MM
通讯作者：
Taketo, MM
影响因子：
24.5
作者：
Shoji, Y;Takahashi, M;Wakabayashi, K
通讯作者：
Wakabayashi, K
DOI：
10.1111/j.1349-7006.1997.tb00337.x
发表时间：
1997-12-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
Nakatsugi, S;Fukutake, M;Wakabayashi, K
通讯作者：
Wakabayashi, K
影响因子：
5.4
作者：
Fukuda, K;Hibiya, Y;Fujiwara, H
通讯作者：
Fujiwara, H
DOI：
10.1016/s1383-5718(00)00096-6
发表时间：
2000-10-10
影响因子：
1.9
作者：
Kaur, IP;Saini, A
通讯作者：
Saini, A