Osteogenic differentiation of mesenchymal stem cells in defined protein beads.
Osteogenic differentiation of mesenchymal stem cells in defined protein beads.
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DOI：
10.1002/jbm.b.31098
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发表时间：
2008-10
影响因子：
3.4
通讯作者：
Stegemann, Jan P.
中科院分区：
文献类型：
--
作者：
Lund, Amanda W.;Bush, Jeff A.;Plopper, George E.;Stegemann, Jan P.
有必要开发用于指导和维持再生医学用人间充质干细胞(HMSC)分化的改进方法。在这里，我们提出了一种方法，将细胞植入特定的蛋白质微环境中，以实现hMSC的定向成骨分化。在HMSC存在下，通过乳化和同步聚合，制备了I型胶原和琼脂糖凝胶复合基质，制备了直径30-150μm的水凝胶“珠子”。培养14-21天，比较珠状环境中人骨髓间充质干细胞的增殖、形态、成骨基因表达和钙沉积与其他二维和三维培养环境的差异。植入40%胶原珠的细胞表现出与凝胶盘中相同的增殖率，但与2D对照组相比，骨涎蛋白表达上调，钙沉积增加。骨钙素基因在3D珠盘中的表达没有改变，而在2D培养中，I型胶原基因的表达相对于细胞下调。水凝胶微珠形式允许受控的细胞分化，是一种细胞输送载体，也可以增强血管侵袭和宿主并入。我们的结果表明，这种微珠的应用可以促进hMSC的成骨表型，这是将其用于骨修复应用的重要一步。
There is a need to develop improved methods for directing and maintaining the differentiation of human mesenchymal stem cells (hMSC) for regenerative medicine. Here, we present a method for embedding cells in defined protein microenvironments for the directed osteogenic differentiation of hMSC. Composite matrices of collagen I and agarose were produced by emulsification and simultaneous polymerization in the presence of hMSC to produce 30–150 μm diameter hydrogel “beads.” The proliferation, morphology, osteogenic gene expression, and calcium deposition of hMSC in bead environments were compared to other two- and three-dimensional culture environments over 14–21 days in culture. Cells embedded within 40% collagen beads exhibited equivalent proliferation rates to those in gel disks, but showed upregulation of bone sialoprotein and increased calcium deposition over 2D controls. Osteocalcin gene expression was not changed in 3D beads and disks, while collagen type I gene expression was downregulated relative to cells in 2D culture. The hydrogel bead format allows controlled cell differentiation and is a cell delivery vehicle that may also enhance vascular invasion and host incorporation. Our results indicate that the application of such beads can be used to promote the osteogenic phenotype in hMSC, which is an important step toward using them in bone repair applications.
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影响因子：
14
作者：
Ehrbar, Martin;Rizzi, Simone C.;Lutolf, Matthias P.
通讯作者：
Lutolf, Matthias P.
影响因子：
14
作者：
Ge, ZG;Baguenard, S;Khor, E
通讯作者：
Khor, E
影响因子：
4.9
作者：
Hosseinkhani, Hossein;Hosseinkhani, Mohsen;Kobayashi, Hisatoshi
通讯作者：
Kobayashi, Hisatoshi
DOI：
10.1136/fn.86.1.f49
发表时间：
2002-01-01
影响因子：
4.4
作者：
Ng, PC;Lam, CWK;Lee, SY
通讯作者：
Lee, SY
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS