Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.
Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors.
复制标题
DOI：
10.3390/ijms22157872
复制
发表时间：
2021-07-23
影响因子：
5.6
通讯作者：
Karnati S
中科院分区：
文献类型：
--
作者：
Meyer MT;Watermann C;Dreyer T;Wagner S;Wittekindt C;Klussmann JP;Ergün S;Baumgart-Vogt E;Karnati S
涎腺癌是一种罕见的侵袭性肿瘤，预后差，缺乏有效的治疗方法。其中腮腺肿瘤占大多数。作为促进细胞氧化还原平衡的代谢机制，过氧化物酶体已成为肿瘤发生的关键参与者。对小鼠和人类细胞的研究已经检查了过氧化物酶体在致癌作用中的作用，结果相互矛盾。这些研究要么检查改变过氧化物酶体增殖物的后果，要么比较它们在健康和肿瘤组织中的表达。然而，没有人专门研究人类腮腺组织中的这种差异，或与过氧化物酶体蛋白及其相关基因表达进行扩展比较。因此，我们研究了不同形态腮腺肿瘤中过氧化物酶体动力学的差异。使用免疫荧光和定量PCR，我们比较了健康和肿瘤腮腺组织样本中关键过氧化物酶体酶和增殖物的表达水平。三种腮腺肿瘤亚型：多形性腺瘤，粘液表皮样癌和腺泡细胞癌进行了检查。我们观察到过氧化物酶体基质蛋白在肿瘤样本中的表达较高，某些酶异常下调;然而，肿瘤亚型之间的表达程度不同。我们的研究结果证实了以前在其他器官组织上的实验结果，并建议过氧化物酶体作为所有或某些腮腺肿瘤亚型的可能治疗靶点或标记物。
Salivary gland cancers are rare but aggressive tumors that have poor prognosis and lack effective cure. Of those, parotid tumors constitute the majority. Functioning as metabolic machinery contributing to cellular redox balance, peroxisomes have emerged as crucial players in tumorigenesis. Studies on murine and human cells have examined the role of peroxisomes in carcinogenesis with conflicting results. These studies either examined the consequences of altered peroxisomal proliferators or compared their expression in healthy and neoplastic tissues. None, however, examined such differences exclusively in human parotid tissue or extended comparison to peroxisomal proteins and their associated gene expressions. Therefore, we examined differences in peroxisomal dynamics in parotid tumors of different morphologies. Using immunofluorescence and quantitative PCR, we compared the expression levels of key peroxisomal enzymes and proliferators in healthy and neoplastic parotid tissue samples. Three parotid tumor subtypes were examined: pleomorphic adenoma, mucoepidermoid carcinoma and acinic cell carcinoma. We observed higher expression of peroxisomal matrix proteins in neoplastic samples with exceptional down regulation of certain enzymes; however, the degree of expression varied between tumor subtypes. Our findings confirm previous experimental results on other organ tissues and suggest peroxisomes as possible therapeutic targets or markers in all or certain subtypes of parotid neoplasms.
登录
查看更多内容
影响因子：
5.8
作者：
Glorieux C;Calderon PB
通讯作者：
Calderon PB
影响因子：
2.3
作者：
Islinger M;Voelkl A;Fahimi HD;Schrader M
通讯作者：
Schrader M
DOI：
10.1007/bf02899685
发表时间：
1992-09-01
期刊：
VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY
影响因子：
--
作者：
CABLE, S;KELLER, JM;DAUCA, M
通讯作者：
DAUCA, M
DOI：
10.1073/pnas.1310894110
发表时间：
2013-09-10
影响因子：
11.1
作者：
Benjamin, Daniel I.;Cozzo, Alyssa;Nomura, Daniel K.
通讯作者：
Nomura, Daniel K.
DOI：
10.1111/j.1349-7006.2001.tb01084.x
发表时间：
2001-02-01
期刊：
JAPANESE JOURNAL OF CANCER RESEARCH
影响因子：
--
作者：
Haga, S;Hinoshita, E;Kuwano, M
通讯作者：
Kuwano, M