The Domestication of a Large DNA Virus by the Wasp Venturia canescens Involves Targeted Genome Reduction through Pseudogenization.
The Domestication of a Large DNA Virus by the Wasp Venturia canescens Involves Targeted Genome Reduction through Pseudogenization.
复制标题
DOI：
10.1093/gbe/evy127
复制
发表时间：
2018-07-01
影响因子：
3.3
通讯作者：
Drezen JM
中科院分区：
文献类型：
--
作者：
Leobold M;Bézier A;Pichon A;Herniou EA;Volkoff AN;Drezen JM
多脱氧核糖核酸病毒（PDVs）是病毒驯化的引人注目的例子，其中黄蜂表达源自染色体整合病毒的大量基因，以产生其繁殖成功所必需的颗粒。寄生蜂通常使用PDV作为毒力基因递送系统，允许在被寄生的宿主体内保护它们的后代。然而，在黄蜂黑星菌（Venturia canescens）中，一个独立的病毒驯化过程导致了另一种策略，因为黄蜂将毒力蛋白掺入称为病毒样颗粒（VLP）的病毒脂质体中，而不是DNA分子。纯化VLP的蛋白质组学分析和转录组测序揭示了一些病毒功能的丧失。特别是，编码衣壳成分的基因不再表达，这解释了为什么VLP不掺入DNA。在这里，对灰葡萄基因组的彻底检查揭示了与大多数涉及功能丧失的基因相对应的假基因的存在。这有力地表明，导致基因特异性假基因化的突变的积累先于在病毒驯化期间观察到的病毒基因的丢失。没有证据表明，共线基因的块丢失，虽然广泛的基因顺序重组的病毒基因组被确定从内源性和外源性病毒之间的比较。这些结果提供了第一个见解的早期阶段的大DNA病毒驯化，涉及大规模的基因组减少，通过基因特异性假基因化，这一过程不同于大的删除描述的细菌内共生体。
Polydnaviruses (PDVs) are compelling examples of viral domestication, in which wasps express a large set of genes originating from a chromosomally integrated virus to produce particles necessary for their reproductive success. Parasitoid wasps generally use PDVs as a virulence gene delivery system allowing the protection of their progeny in the body of parasitized host. However, in the wasp Venturia canescens an independent viral domestication process led to an alternative strategy as the wasp incorporates virulence proteins in viral liposomes named virus-like particles (VLPs), instead of DNA molecules. Proteomic analysis of purified VLPs and transcriptome sequencing revealed the loss of some viral functions. In particular, the genes coding for capsid components are no longer expressed, which explains why VLPs do not incorporate DNA. Here a thorough examination of V. canescens genome revealed the presence of the pseudogenes corresponding to most of the genes involved in lost functions. This strongly suggests that an accumulation of mutations that leads to gene specific pseudogenization precedes the loss of viral genes observed during virus domestication. No evidence was found for block loss of collinear genes, although extensive gene order reshuffling of the viral genome was identified from comparisons between endogenous and exogenous viruses. These results provide the first insights on the early stages of large DNA virus domestication implicating massive genome reduction through gene-specific pseudogenization, a process which differs from the large deletions described for bacterial endosymbionts.
登录
查看更多内容
影响因子：
64.5
作者：
Ashley J;Cordy B;Lucia D;Fradkin LG;Budnik V;Thomson T
通讯作者：
Thomson T
影响因子：
6.7
作者：
Burke GR;Thomas SA;Eum JH;Strand MR
通讯作者：
Strand MR
影响因子：
5.4
作者：
Cheng, Ruo-Lin;Xi, Yu;Zhang, Chuan-Xi
通讯作者：
Zhang, Chuan-Xi
影响因子：
3.8
作者：
CRAWFORD, AM;SHEEHAN, C
通讯作者：
SHEEHAN, C
DOI：
10.1098/rstb.2013.0047
发表时间：
2013-09-19
影响因子：
6.3
作者：
Bezier, Annie;Louis, Faustine;Drezen, Jean-Michel
通讯作者：
Drezen, Jean-Michel