Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions.
Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions.
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DOI：
10.1038/s42003-021-01901-z
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发表时间：
2021-03-19
影响因子：
5.9
通讯作者：
Vervelde L
中科院分区：
文献类型：
--
作者：
Nash TJ;Morris KM;Mabbott NA;Vervelde L
哺乳动物三维（3D）肠样体反映了体内肠道组织，是研究肠道细胞生物学和宿主-病原体相互作用的有力工具。我们从鸡的肠胚绒毛和成体隐窝中发育出复杂的多分叶三维小肠。这些鸟类肠样体在没有哺乳动物肠样体所需的结构支持的情况下在悬浮状态下发育最佳，导致由内而外的肠样构象具有面向媒体的顶端刷状边界。组织学和转录分析表明，这些肠样细胞由细胞间连接结合的分化肠上皮细胞组成，特别是包括上皮内白细胞和固有层白细胞的内核。这些肠道的有利极化使得鼠伤寒沙门氏菌、甲型流感病毒和柔嫩艾美耳球虫在不需要微注射的情况下感染上皮顶端表面。我们已经建立了一个全面的鸡肠模型，该模型具有探索宿主-病原体，食品科学和制药研究中上皮和白细胞相互作用和反应的潜力。Nash等人从肠胚绒毛和成虫隐窝中开发并表征了多分叶三维鸡肠，这构成了一个可接近的鸡肠模型。该模型可以在不需要显微注射的情况下进行病毒感染等操作，从而为探索宿主-病原体，食品科学和药物研究中的上皮和白细胞相互作用和反应提供更多机会。
Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host–pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological and transcriptional analyses show these enteroids comprise of differentiated intestinal epithelial cells bound by cell-cell junctions, and notably, include intraepithelial leukocytes and an inner core of lamina propria leukocytes. The advantageous polarisation of these enteroids has enabled infection of the epithelial apical surface with Salmonella Typhimurium, influenza A virus and Eimeria tenella without the need for micro-injection. We have created a comprehensive model of the chicken intestine which has the potential to explore epithelial and leukocyte interactions and responses in host–pathogen, food science and pharmaceutical research. Nash et al. develop and characterise multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts, which constitute an accessible model of the chicken intestine. This model enables manipulations, such as viral infection, to be performed without the need for micro-injection, thus opening up more opportunities to explore epithelial and leukocyte interactions and responses in host–pathogen, food science and pharmaceutical research.
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