Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
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DOI：
10.1002/pmic.200900573
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发表时间：
2010-02
期刊：
影响因子：
3.4
通讯作者：
Loo, Joseph A.
中科院分区：
文献类型：
--
作者：
Kang, Xuedong;Li, Ning;Wang, Meiying;Boontheung, Pinmanee;Sioutas, Constantinos;Harkema, Jack R.;Bramble, Lori A.;Nel, Andre E.;Loo, Joseph A.
关键词：
来自空气污染的环境颗粒物（PM）与哮喘的加重有关。PM的佐剂作用的免疫学基础尚不清楚。活性氧（ROS）的产生和由此产生的氧化应激已被确定为主要机制之一。使用一种新的鼻内致敏模型，在该模型中，环境PM被用作佐剂来增强过敏性炎症(Li等人，Environ。《健康展望》，2009,117,1116-1123)，应用蛋白质组学方法研究环境PM的辅助作用。超细颗粒（UFP）的体内辅助作用的增强与体外氧化电位和氧化还原循环有机化学物质含量的增加有关。正常小鼠和致敏小鼠支气管肺泡灌洗液蛋白采用双向凝胶电泳分离，质谱法鉴定。聚合免疫球蛋白受体、补体C3、中性粒细胞明胶酶相关脂质蛋白、几丁质酶-3样蛋白3、几丁质酶-3样蛋白4和酸性哺乳动物几丁质酶被多环芳烃（PAH）含量和更高氧化电位的UFP显著上调。这些蛋白可能是空气污染中PM通过在免疫系统中产生ROS而靶向的重要特异性元素，并可能参与过敏原致敏和哮喘发病机制。
Ambient particulate matter (PM) from air pollution is associated with exacerbation of asthma. The immunological basis for the adjuvant effects of PM is still not well understood. The generation of reactive oxygen species (ROS) and the resulting oxidative stress has been identified as one of the major mechanisms. Using a new intranasal sensitization model in which ambient PM is used as an adjuvant to enhance allergic inflammation (Li et al., Environ. Health Perspect. 2009, 117, 1116-1123), a proteomics approach was applied to study the adjuvant effects of ambient PM. The enhanced in vivo adjuvant effect of ultrafine particles (UFP) correlates with a higher in vitro oxidant potential and a higher content of redox-cycling organic chemicals. Bronchoalveolar lavage fluid proteins from normal and sensitized mice were resolved by two-dimensional gel electrophoresis, and identified by mass spectrometry. Polymeric immunoglobulin receptor, complement C3, neutrophil gelatinase-associated lipocalin, chitinase-3-like protein 3, chitinase-3-like protein 4, and acidic mammalian chitinase demonstrated significantly enhanced up-regulation by UFP with a polycyclic aromatic hydrocarbon (PAH) content and a higher oxidant potential. These proteins may be the important specific elements targeted by PM in air pollution through the ability to generate ROS in the immune system, and may be involved in allergen sensitization and asthma pathogenesis.
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影响因子：
10.4
作者：
Li N;Wang M;Bramble LA;Schmitz DA;Schauer JJ;Sioutas C;Harkema JR;Nel AE
通讯作者：
Nel AE
影响因子：
9
作者：
ALEXANDER, JW;OGLE, CK;MACMILLAN, BG
通讯作者：
MACMILLAN, BG
影响因子：
14.2
作者：
Chan, Ray Chun-Fai;Wang, Meiying;Nel, Andre E.
通讯作者：
Nel, Andre E.
影响因子：
8.6
作者：
Matsumoto, Aki;Hiramatsu, Kumiko;Sugawara, Isamu
通讯作者：
Sugawara, Isamu
影响因子：
14.2
作者：
Hao, MQ;Comier, S;Nel, A
通讯作者：
Nel, A