Transcriptome Analysis of Pyrus betulaefolia Seedling Root Responses to Short-Term Potassium Deficiency.
Transcriptome Analysis of Pyrus betulaefolia Seedling Root Responses to Short-Term Potassium Deficiency.
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杜梨幼苗根对短期缺钾反应的转录组分析
DOI：
10.3390/ijms21228857
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发表时间：
2020-11-23
影响因子：
5.6
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Yang H;Li Y;Jin Y;Kan L;Shen C;Malladi A;Nambeesan S;Xu Y;Dong C
钾在植物的多种生理发育过程中起着至关重要的作用。它的缺乏是一种常见的抑制植物生长和降低作物生产力的非生物胁迫。更好地了解植物对低钾胁迫的响应机制有助于提高植物对钾的利用效率。然而，在梨（Pyrus sp）等果树物种中，这种反应的特征仍然很差。本研究分析了常用梨砧木白桦梨（Pyrus betulaefolia）对缺钾胁迫（0 mM）的生理和转录组反应。缺钾导致根系形态发生明显变化，短期低钾胁迫导致根系生长迅速增强。转录组分析表明，在缺钾后6小时内，根转录组发生了协调改变，这一过程持续到处理后15 d。缺钾导致高亲和K+转运蛋白PbHAK5 （Pbr037826.1）的表达增加（高达5倍），表明与K+获取相关的机制上调。缺钾胁迫下根系生长的增强与转录因子PbMYB44 （Pbr015309.1）表达的快速和持续下降有关，PbMYB44可能参与介导生长素反应，以及与调节根系生长相关的多个基因表达的增加。缺钾处理显著提高了植物激素吲哚乙酸（IAA）、ABA、ETH、赤霉素（GA3）和茉莉酸（JA）的浓度。此外，在缺钾条件下，与碳代谢相关的酶如SORBITOL DEHYDROGENASE （SDH）和蔗糖合成酶（SUS）的编码基因在根系中的表达显著增强，这可能表明代谢增强以满足生长和K+获取所需的能量需求。总之，这些数据表明，白桦缺钾导致转录组快速重编程，以促进根生长和K+获取。这些数据为了解梨和其他相关果树的低钾耐受性机制提供了关键的分子基础，并确定了值得进一步分析的潜在候选植物。
Potassium (K) plays a crucial role in multiple physiological and developmental processes in plants. Its deficiency is a common abiotic stress that inhibits plant growth and reduces crop productivity. A better understanding of the mechanisms involved in plant responses to low K could help to improve the efficiency of K use in plants. However, such responses remain poorly characterized in fruit tree species such as pears (Pyrus sp). We analyzed the physiological and transcriptome responses of a commonly used pear rootstock, Pyrus betulaefolia, to K-deficiency stress (0 mM). Potassium deprivation resulted in apparent changes in root morphology, with short-term low-K stress resulting in rapidly enhanced root growth. Transcriptome analyses indicated that the root transcriptome was coordinately altered within 6 h after K deprivation, a process that continued until 15 d after treatment. Potassium deprivation resulted in the enhanced expression (up to 5-fold) of a putative high-affinity K+ transporter, PbHAK5 (Pbr037826.1), suggesting the up-regulation of mechanisms associated with K+ acquisition. The enhanced root growth in response to K-deficiency stress was associated with a rapid and sustained decrease in the expression of a transcription factor, PbMYB44 (Pbr015309.1), potentially involved in mediating auxin responses, and the increased expression of multiple genes associated with regulating root growth. The concentrations of several phytohormones including indoleacetic acid (IAA), ABA, ETH, gibberellin (GA3), and jasmonic acid (JA) were higher in response to K deprivation. Furthermore, genes coding for enzymes associated with carbon metabolism such as SORBITOL DEHYDROGENASE (SDH) and SUCROSE SYNTHASE (SUS) displayed greatly enhanced expression in the roots under K deprivation, presumably indicating enhanced metabolism to meet the increased energy demands for growth and K+ acquisition. Together, these data suggest that K deprivation in P. betulaefolia results in the rapid re-programming of the transcriptome to enhance root growth and K+ acquisition. These data provide key insights into the molecular basis for understanding low-K-tolerance mechanisms in pears and in other related fruit trees and identifying potential candidates that warrant further analyses.
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影响因子：
7.2
作者：
Papdi, Csaba;Perez-Salamo, Imma;Szabados, Laszlo
通讯作者：
Szabados, Laszlo
影响因子：
7.2
作者：
Cellier, F;Conéjéro, G;Casse, F
通讯作者：
Casse, F
影响因子：
4.6
作者：
Gupta A;Hisano H;Hojo Y;Matsuura T;Ikeda Y;Mori IC;Senthil-Kumar M
通讯作者：
Senthil-Kumar M
影响因子：
1.7
作者：
He, Yanqiu;Bose, Santosh Kumar;Yin, Heng
通讯作者：
Yin, Heng
影响因子：
7.4
作者：
Gierth, M;Mäser, P;Schroeder, JI
通讯作者：
Schroeder, JI