Kinetochores generate microtubules with distal plus ends: their roles and limited lifetime in mitosis.
Kinetochores generate microtubules with distal plus ends: their roles and limited lifetime in mitosis.
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DOI：
10.1016/j.devcel.2009.12.018
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发表时间：
2010-02-16
影响因子：
11.8
通讯作者：
Tanaka TU
中科院分区：
文献类型：
--
作者：
Kitamura E;Tanaka K;Komoto S;Kitamura Y;Antony C;Tanaka TU
在早期有丝分裂中，微管可以在着丝粒和纺锤体极处产生。然而，着丝粒衍生的微管的作用和调节尚不清楚。一般来说，中期纺锤体微管的定向使得它们的正端与动粒结合。然而，我们现在有证据表明，在芽殖酵母的早期有丝分裂过程中，微管在具有远端正端的动粒处产生。这些动粒衍生的微管沿其长度与从纺锤极延伸的微管相互作用，促进动粒加载到纺锤极微管的侧面。一旦着丝粒被加载，着丝粒上就不再产生微管，而剩下的微管迅速消失，对中期纺锤体没有贡献。 Stu2（脊椎动物 XMAP215/ch-TOG 的直系同源物）定位于着丝粒，并在调节着丝粒衍生的微管中发挥核心作用。我们的工作提供了对着丝粒微管生成以及促进着丝粒与纺锤体极微管初始相互作用的机制的深入了解。 ► 短命的着丝粒衍生微管 (MT) 群体具有远端正端 ► 这些 MT 在早期有丝分裂中产生并受 Stu2 调节 ► 它们在着丝粒附着之前与纺锤体极衍生的 MT 相互作用 ► 一旦着丝粒附着到纺锤体极 MT，短命的 MT 群体就会消失
In early mitosis, microtubules can be generated at kinetochores as well as at spindle poles. However, the role and regulation of kinetochore-derived microtubules have been unclear. In general, metaphase spindle microtubules are oriented such that their plus ends bind to kinetochores. However, we now have evidence that, during early mitosis in budding yeast, microtubules are generated at kinetochores with distal plus ends. These kinetochore-derived microtubules interact along their length with microtubules that extend from a spindle pole, facilitating kinetochore loading onto the lateral surface of spindle pole microtubules. Once kinetochores are loaded, microtubules are no longer generated at kinetochores, and those that remain disappear rapidly and do not contribute to the metaphase spindle. Stu2 (the ortholog of vertebrate XMAP215/ch-TOG) localizes to kinetochores and plays a central role in regulating kinetochore-derived microtubules. Our work provides insight into microtubule generation at kinetochores and the mechanisms that facilitate initial kinetochore interaction with spindle pole microtubules. ► A short-lived population of kinetochore-derived microtubules (MTs) has distal plus ends ► These MTs are generated in early mitosis and regulated by Stu2 ► They interact with spindle pole-derived MTs before kinetochore attachment ► Once kinetochores attach to spindle-pole MTs, the short-lived MT population disappears
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DOI：
10.1083/jcb.84.1.151
发表时间：
1980-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bergen LG;Kuriyama R;Borisy GG
通讯作者：
Borisy GG
影响因子：
21.3
作者：
Gupta, Mohan L., Jr.;Carvalho, Pedro;Pellman, David
通讯作者：
Pellman, David
影响因子：
7.5
作者：
Schiebel, E
通讯作者：
Schiebel, E
影响因子：
11.4
作者：
Maekawa, H;Usui, T;Schiebel, E
通讯作者：
Schiebel, E
影响因子：
64.8
作者：
Tanaka, K;Mukae, N;Tanaka, TU
通讯作者：
Tanaka, TU