Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.
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DOI：
10.1371/journal.pone.0038954
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Cyr TD
中科院分区：
文献类型：
--
作者：
Rosu-Myles M;She YM;Fair J;Muradia G;Mehic J;Menendez P;Prasad SS;Cyr TD
骨髓基质细胞培养物含有多能细胞，可能具有组织修复的治疗效用;然而，维持这种功能的细胞的特性仍然很差。我们利用一种独特的小鼠骨髓基质模型，结合液相色谱质谱法，比较了多能（MSC）（CD 105+）和非多能（CD 105 −）基质细胞的核、细胞质和膜相关蛋白质组。在25个最可靠的鉴定的蛋白质中，有10个通过实时PCR和Western Blot验证为在CD 105+细胞中高度富集，并且是不同生物学途径和功能网络的成员。这些蛋白质中的五种也被鉴定为在来源于标准和无血清人基质培养物的人MSC中潜在表达。在人基质细胞中鉴定的每种蛋白质的定量仅受培养基条件的影响最小，但在骨髓供体之间差异很大。这项研究提供了进一步的证据，培养的骨髓基质细胞之间的异质性，并确定潜在的候选蛋白质，可能被证明是有用的识别和定量小鼠和人MSC在体外。
Bone marrow stromal cell cultures contain multipotent cells that may have therapeutic utility for tissue restoration; however, the identity of the cell that maintains this function remains poorly characterized. We have utilized a unique model of murine bone marrow stroma in combination with liquid chromatography mass spectrometry to compare the nuclear, cytoplasmic and membrane associated proteomes of multipotent (MSC) (CD105+) and non-multipotent (CD105−) stromal cells. Among the 25 most reliably identified proteins, 10 were verified by both real-time PCR and Western Blot to be highly enriched, in CD105+ cells and were members of distinct biological pathways and functional networks. Five of these proteins were also identified as potentially expressed in human MSC derived from both standard and serum free human stromal cultures. The quantitative amount of each protein identified in human stromal cells was only minimally affected by media conditions but varied highly between bone marrow donors. This study provides further evidence of heterogeneity among cultured bone marrow stromal cells and identifies potential candidate proteins that may prove useful for identifying and quantifying both murine and human MSC in vitro.
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影响因子：
7.8
作者：
Asakura, Atsushi;Seale, Patrick;Girgis-Gabardo, Adele;Rudnicki, Michael A
通讯作者：
Rudnicki, Michael A
DOI：
10.1073/pnas.0608249103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Lee, Ryang Hwa;Seo, Min Jeong;Prockop, Darwin J.
通讯作者：
Prockop, Darwin J.
影响因子：
5.5
作者：
Germani, Antonia;Limana, Federica;Capogrossi, Maurizio C.
通讯作者：
Capogrossi, Maurizio C.
影响因子：
5.4
作者：
Lax, Sian;Hardie, Debbie L.;Wilson, Amy;Douglas, Mike R.;Anderson, Graham;Huso, David;Isacke, Clare M.;Buckley, Christopher D.
通讯作者：
Buckley, Christopher D.
DOI：
10.1111/j.1365-2184.1970.tb00347.x
发表时间：
1970-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
通讯作者：
LALYKINA, KS