Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro.
Regulation of carotenoid accumulation and the expression of carotenoid metabolic genes in citrus juice sacs in vitro.
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DOI：
10.1093/jxb/err318
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发表时间：
2012-01
影响因子：
6.9
通讯作者：
Nesumi H
中科院分区：
文献类型：
--
作者：
Zhang L;Ma G;Kato M;Yamawaki K;Takagi T;Kiriiwa Y;Ikoma Y;Matsumoto H;Yoshioka T;Nesumi H
在本研究中，为了研究调节柑橘类胡萝卜素积累的机制，利用萨摩柑橘（Citrus unshiu Marc.）、瓦伦西亚橙（Citrus sinensis Osbeck）和里斯本柠檬（Citrus limon Burm.f.）三个柑橘品种的汁囊建立了体外培养系统。随着类胡萝卜素的积累，三个品种的汁囊逐渐增大。 3个品种离体汁囊中类胡萝卜素含量的变化规律和类胡萝卜素代谢基因的表达与树上成熟相似。使用该系统，研究了类胡萝卜素含量和类胡萝卜素代谢基因表达对环境刺激的反应的变化。结果表明，三个品种的类胡萝卜素积累受到蓝光处理的诱导，而不受红光处理的影响。 CitPSY 表达的不同调节（受蓝光上调而不受红光影响）导致了萨摩柑橘和瓦伦西亚橙中类胡萝卜素含量的不同变化。在所有三个品种中，蔗糖和甘露醇处理后都观察到类胡萝卜素含量增加。然而，两种处理中类胡萝卜素的积累受到转录水平上不同机制的调节。通过脱落酸 (ABA) 处理，本研究中研究的基因表达在萨摩柑橘和里斯本柠檬中上调，表明 ABA 在转录水平上诱导其自身的生物合成。 ABA 的这种反馈调节导致类胡萝卜素含量减少。经过赤霉素（GA）处理，三个品种的类胡萝卜素含量显着下降。 GA 处理后，三个品种中类胡萝卜素代谢相关基因的表达变化各不相同。这些结果为改善柑橘在果实成熟过程中的类胡萝卜素含量和成分提供了见解。
In the present study, to investigate the mechanisms regulating carotenoid accumulation in citrus, a culture system was set up in vitro with juice sacs of three citrus varieties, Satsuma mandarin (Citrus unshiu Marc.), Valencia orange (Citrus sinensis Osbeck), and Lisbon lemon (Citrus limon Burm.f.). The juice sacs of all the three varieties enlarged gradually with carotenoid accumulation. The changing patterns of carotenoid content and the expression of carotenoid metabolic genes in juice sacs in vitro were similar to those ripening on trees in the three varieties. Using this system, the changes in the carotenoid content and the expression of carotenoid metabolic genes in response to environmental stimuli were investigated. The results showed that carotenoid accumulation was induced by blue light treatment, but was not affected by red light treatment in the three varieties. Different regulation of CitPSY expression, which was up-regulated by blue light while unaffected by red light, led to different changes in carotenoid content in response to these two treatments in Satsuma mandarin and Valencia orange. In all three varieties, increases in carotenoid content were observed with sucrose and mannitol treatments. However, the accumulation of carotenoid in the two treatments was regulated by distinct mechanisms at the transcriptional level. With abscisic acid (ABA) treatment, the expression of the genes investigated in this study was up-regulated in Satsuma mandarin and Lisbon lemon, indicating that ABA induced its own biosynthesis at the transcriptional level. This feedback regulation of ABA led to decreases in carotenoid content. With gibberellin (GA) treatment, carotenoid content was significantly decreased in the three varieties. Changes in the expression of genes related to carotenoid metabolism varied among the three varieties in response to GA treatment. These results provided insights into improving carotenoid content and composition in citrus during fruit maturation.
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影响因子：
6.1
作者：
Goodner, KL;Rouseff, RL;Hofsommer, HJ
通讯作者：
Hofsommer, HJ
影响因子：
7.4
作者：
HUFF, A
通讯作者：
HUFF, A
影响因子：
7.4
作者：
Alba, R;Cordonnier-Pratt, MM;Pratt, LH
通讯作者：
Pratt, LH
影响因子：
6.9
作者：
Alquézar B;Zacarías L;Rodrigo MJ
通讯作者：
Rodrigo MJ
影响因子：
6.9
作者：
Burbidge, A;Grieve, T;Taylor, I
通讯作者：
Taylor, I