Neural cell 3D microtissue formation is marked by cytokines' up-regulation.
Neural cell 3D microtissue formation is marked by cytokines' up-regulation.
复制标题
DOI：
10.1371/journal.pone.0026821
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kisaalita WS
中科院分区：
文献类型：
--
作者：
Lai Y;Asthana A;Cheng K;Kisaalita WS
Cells cultured in three dimensional (3D) scaffolds as opposed to traditional two-dimensional (2D) substrates have been considered more physiologically relevant based on their superior ability to emulate the in vivo environment. Combined with stem cell technology, 3D cell cultures can provide a promising alternative for use in cell-based assays or biosensors in non-clinical drug discovery studies. To advance 3D culture technology, a case has been made for identifying and validating three-dimensionality biomarkers. With this goal in mind, we conducted a transcriptomic expression comparison among neural progenitor cells cultured on 2D substrates, 3D porous polystyrene scaffolds, and as 3D neurospheres (in vivo surrogate). Up-regulation of cytokines as a group in 3D and neurospheres was observed. A group of 13 cytokines were commonly up-regulated in cells cultured in polystyrene scaffolds and neurospheres, suggesting potential for any or a combination from this list to serve as three-dimensionality biomarkers. These results are supportive of further cytokine identification and validation studies with cells from non-neural tissue.
登录
查看更多内容
DOI：
10.1073/pnas.0808932106
发表时间：
2009-01-13
影响因子：
11.1
作者：
Fischbach, Claudia;Kong, Hyun Joon;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
14
作者：
Klapperich, CM;Bertozzi, CR
通讯作者：
Bertozzi, CR
影响因子：
4.4
作者：
Ito, Y;Yamamoto, M;Sobue, G
通讯作者：
Sobue, G
影响因子：
4.8
作者：
Calzolari, Filippo;Appolloni, Irene;Malatesta, Paolo
通讯作者：
Malatesta, Paolo