Detection of nisin and fibrinogen adsorption on poly(ethylene oxide) coated polyurethane surfaces by time-of-flight secondary ion mass spectrometry (TOF-SIMS).
Detection of nisin and fibrinogen adsorption on poly(ethylene oxide) coated polyurethane surfaces by time-of-flight secondary ion mass spectrometry (TOF-SIMS).
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DOI：
10.1016/j.jcis.2011.03.014
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发表时间：
2011-06-01
影响因子：
9.9
通讯作者：
McGuire, Joseph
中科院分区：
文献类型：
--
作者：
Schilke, Karl F.;McGuire, Joseph
关键词：
通过PEO-聚丁二烯-PEO三嵌段表面活性剂的吸附和γ-辐照，在医用级Pellethane®和Tygon®聚氨酯表面上形成稳定的悬垂聚环氧乙烷（PEO）层。涂覆和未涂覆的聚氨酯分别或依次挑战乳链菌肽（一种具有抗微生物活性的小多肽）和/或纤维蛋白原，然后用飞行时间二次离子质谱法（TOF-SIMS）进行分析。通过稳健的主成分分析（PCA）的数据减少允许检测离群值，并区分吸附的乳链菌肽和纤维蛋白原。纤维蛋白原接触的表面，有或没有乳酸链球菌素，是非常相似的未涂覆的聚合物表面，与几乎完全取代或覆盖以前吸附的乳酸链球菌素纤维蛋白原一致。相比之下，乳酸链球菌素加载的PEO层基本上保持不变的挑战后，与纤维蛋白原，这表明吸附的乳酸链球菌素是稳定的悬垂PEO层内，而肽加载的PEO层保留其排斥大蛋白质的能力。在医用聚合物上负载有治疗性多肽的PEO涂层具有用于产生可植入或血液接触装置的防污和生物功能表面的潜力。
Stable, pendant polyethylene oxide (PEO) layers were formed on medical-grade Pellethane® and Tygon® polyurethane surfaces, by adsorption and gamma-irradiation of PEO-polybutadiene-PEO triblock surfactants. Coated and uncoated polyurethanes were challenged individually or sequentially with nisin (a small polypeptide with antimicrobial activity) and/or fibrinogen, and then analyzed with time-of-flight secondary ion mass spectrometry (TOF-SIMS). Data reduction by robust principal components analysis (PCA) allowed detection of outliers, and distinguished adsorbed nisin and fibrinogen. Fibrinogen-contacted surfaces, with or without nisin, were very similar on uncoated polymer surfaces, consistent with nearly complete displacement or coverage of previously-adsorbed nisin by fibrinogen. In contrast, nisin-loaded PEO layers remained essentially unchanged upon challenge with fibrinogen, suggesting that the adsorbed nisin is stabilized within the pendant PEO layer, while the peptide-loaded PEO layer retains its ability to repel large proteins. Coatings of PEO loaded with therapeutic polypeptides on medical polymers have the potential to be used to produce anti-fouling and biofunctional surfaces for implantable or blood-contacting devices.
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