Inhibition of leucine aminopeptidase 3 suppresses invasion of ovarian cancer cells through down-regulation of fascin and MMP-2/9.
Inhibition of leucine aminopeptidase 3 suppresses invasion of ovarian cancer cells through down-regulation of fascin and MMP-2/9.
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抑制亮氨酸氨肽酶 3 通过下调肌成束蛋白和 MMP-2/9 抑制卵巢癌细胞的侵袭。
DOI：
10.1016/j.ejphar.2015.10.039
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发表时间：
2015-12
影响因子：
5
通讯作者：
Fang Chunyan
中科院分区：
文献类型：
--
作者：
Wang Xuejian;Shi Lihong;Deng Yilin;Qu Meihua;Mao Shumei;Xu Liyan;Xu Wenfang;Fang Chunyan
Leucine aminopeptidase 3 (LAP3) is a cell surface aminopeptidase that catalyzes the hydrolysis of leucine residues from the amino termini of protein or peptide substrates. The over-expression of LAP3 correlates with prognosis and malignant development of several human cell carcinomas. However, the molecular mechanism remains unknown. In this study, we used ES-2 ovarian cancer cell line as a model system to explore the role of LAP3 in regulation of cancer cell invasion by employing a natural LAP3 inhibitor bestatin and LAP3 siRNA. Bestatin inhibited tumor cell migration and invasion in a dose-dependent manner. More interestingly, bestatin down-regulated expression of fascin protein and inhibited activity of fascin promoter luciferase reporter. Both proteome profiler array and Western blot assay showed that bestatin up-regulated the phosphorylation of Hsp27. Furthermore, LAP3 siRNA could up-regulate the phosphorylation of Hsp27 and down-regulate the expression of fascin. Meanwhile, LAP3 siRNA could also down-regulate the phosphorylation of Akt and the expression of MMP-2/9. Taken together, LAP3 could affect the expression of fascin and MMP-2/9 and may act as a potential anti-metastasis therapeutic target.
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DOI：
10.1083/jcb.200603013
发表时间：
2006-09-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Vignjevic D;Kojima S;Aratyn Y;Danciu O;Svitkina T;Borisy GG
通讯作者：
Borisy GG
影响因子：
4.8
作者：
Sun, Jianwei;He, Huifang;Yang, Shengyu
通讯作者：
Yang, Shengyu
影响因子：
1.4
作者：
Siyuan Tian;S. Chen;B. Shao;H. Cai;Yuan Zhou;Yilong Zhou;Ai-bing Xu
通讯作者：
Siyuan Tian;S. Chen;B. Shao;H. Cai;Yuan Zhou;Yilong Zhou;Ai-bing Xu
LAP3通过调节胶质瘤细胞的增殖、迁移和侵袭促进胶质瘤进展
DOI：
10.1016/j.ijbiomac.2014.10.021
发表时间：
2015-01-01
影响因子：
8.2
作者：
He, Xiaojuan;Huang, Qingfeng;Wang, Dongling
通讯作者：
Wang, Dongling
影响因子：
4.8
作者：
Li, Dan;Jin, Lingtao;Kang, Sumin
通讯作者：
Kang, Sumin