Molecular cloning and expression analysis of SpWRKY6 gene from Solanum pimpinellifolium
Molecular cloning and expression analysis of SpWRKY6 gene from Solanum pimpinellifolium
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菠菜SpWRKY6基因的分子克隆及表达分析
DOI：
10.1007/s10535-016-0582-x
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发表时间：
2016-02
影响因子：
1.5
通讯作者：
Li, J. B.
中科院分区：
文献类型：
--
作者：
Liu, Z.;Luan, Y. S.;Li, J. B.
转录因子WRKY在植物对生物和非生物胁迫的应答中起着重要作用，以往的研究主要集中在模式植物上，对番茄的研究非常有限。本研究利用电子克隆和RT-PCR方法从番茄抗晚疫病品种L3708中克隆到一个新的病原菌诱导的WRKY基因，命名为SpWRKY 6。与其它植物WRKY基因序列比对表明，SpWRKY 6含有两个WRKY结构域，属于Ⅰ类WRKY转录因子。此外，在该基因的启动子区还发现了一些与环境胁迫应答相关的顺式作用元件。通过使用Genevestigator工具分析番茄中的SpWRKY 6和拟南芥中的一个orthopathic基因的微阵列数据来确定基因表达模式。结果表明，该基因具有很强的生物和非生物胁迫响应行为。此外，生物信息学结果通过真实的时间定量聚合酶链反应证实，SpWRKY 6分别在致病疫霉和灰葡萄孢侵染后被快速诱导表达。SpWRKY 6的表达上调应用各种植物激素，包括水杨酸，茉莉酸甲酯，脱落酸。同样，NaCl、干旱、热、冷和HgCl 2处理诱导SpWRKY 6表达。
Ttranscription factors WRKY play vital roles in response to biotic and abiotic stresses, and previous studies have predominantly focused on model plants and fairly limited research has been performed with tomato. In the present study, a novel pathogen-induced WRKY gene named SpWRKY6 was isolated from the late blight resistant tomato (Solanum pimpinellifolium) cultivar L3708 using in silico cloning and reverse transcription polymerase chain reaction (RT-PCR) methods. Multiple sequence alignment with other plant WRKYs indicates that SpWRKY6 contains two WRKY domains and belongs to group I WRKY transcription factors. Furthermore, some cis-acting elements associated with responses to environmental stresses were observed in the promoter region of this gene. Gene expression patterns were determined by analyzing microarray data of SpWRKY6 in tomato and of an orthologous gene from Arabidopsis thaliana using the Genevestigator tool. The results reveal a very strong biotic and abiotic stress responsive behaviour of this gene. Moreover, bioinformatics results were confirmed by real time quantitative polymerase chain reaction and show that SpWRKY6 expression was rapidly induced after infection with Phytophthora infestans and Botrytis cinerea, respectively. Expression of SpWRKY6 was up-regulated by application of various phytohormones including salicylic acid, methyl jasmonate, and abscisic acid. Likewise, the SpWRKY6 expression was induced by NaCl, drought, heat, cold, and HgCl2 treatments.
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影响因子：
2.1
作者：
Ding B;Wang J;Song N;Li M;Cheng Q;Huang G;Guo Y;Fu Y;Xie C;Sun Q;Xie X
通讯作者：
Xie X
影响因子：
11.6
作者：
R. Quatrano
通讯作者：
R. Quatrano
影响因子：
7.2
作者：
Bhattarai, Kishor K.;Atamian, Hagop S.;Eulgem, Thomas
通讯作者：
Eulgem, Thomas
影响因子：
1.5
作者：
X. Ren;W. D. Huang;W. Z. Li;D. Yu
通讯作者：
X. Ren;W. D. Huang;W. Z. Li;D. Yu
影响因子：
3.5
作者：
Yanxu Yin;Shu-Bin Wang;Huai-Xia Zhang;Huaijuan Xiao;Jing-Hao Jin;J-J Ji-J;Hua Jing;Rugang Chen
通讯作者：
Yanxu Yin;Shu-Bin Wang;Huai-Xia Zhang;Huaijuan Xiao;Jing-Hao Jin;J-J Ji-J;Hua Jing;Rugang Chen