Dried urine spots for detection and quantification of cytomegalovirus in newborns.
Dried urine spots for detection and quantification of cytomegalovirus in newborns.
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DOI：
10.1016/j.diagmicrobio.2012.04.009
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发表时间：
2012-08
影响因子：
2.9
通讯作者：
Arav-Boger, Ravit
中科院分区：
文献类型：
--
作者：
Forman, Michael;Valsamakis, Alexandra;Arav-Boger, Ravit
关键词：
据报道，尿干斑 (DUS) 为先天性巨细胞病毒 (CMV) 感染提供了一种简单的筛查工具。我们开发了一种 DUS 定量 CMV 的标准化方法。两次应用 20 μL 尿液保留在滤纸盘的边缘内，用于确定掺入尿液并施加在滤纸盘上的 Towne CMV 的分析性能。可测量范围为 3.7 至 ≥8.0 log10 拷贝/mL。检测限为 22 个 DNA 拷贝/盘。先天感染新生儿和阴性对照的尿液样本按 1:10 稀释或以相同体积涂在滤纸上。 DUS 的 DNA 拷贝数与 1:10 稀释尿液的 DNA 拷贝数相关性良好，但 DUS 的 DNA 拷贝数有较低的趋势（0.3 log10 差异）。我们的 CMV 检测和定量标准化方法可以促进资源有限地区的 CMV 研究，并允许对接受治疗的婴儿的病毒载量进行纵向监测。
Dried urine spots (DUS) have been reported to provide a simple screening tool for congenital cytomegalovirus (CMV) infection. We developed a standardized method for CMV quantification from DUS. Two applications of 20 μ L urine remained within the rim of the filter paper disc and were used to determine the analytical performance of Towne CMV spiked into urine and applied on the discs. The measurable range spanned 3.7 to ≥8.0 log10 copies/mL. The detection limit was 22 DNA copies/disc. Urine samples from congenitally-infected newborns and negative controls were either diluted 1:10 or applied on filter paper at the same volume. DNA copy number from DUS correlated well with copy number from 1:10 diluted urine, although there was a trend for lower levels from DUS (0.3 log10 difference). Our standardized method for CMV detection and quantification may facilitate CMV studies in resource-limited areas and allow for longitudinal monitoring of viral loads in treated infants.
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