Bacterial lysine decarboxylase influences human dental biofilm lysine content, biofilm accumulation, and subclinical gingival inflammation.
Bacterial lysine decarboxylase influences human dental biofilm lysine content, biofilm accumulation, and subclinical gingival inflammation.
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DOI：
10.1902/jop.2011.110474
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发表时间：
2012-08
影响因子：
4.3
通讯作者：
Levine M
中科院分区：
文献类型：
--
作者：
Lohinai Z;Keremi B;Szoko E;Tabi T;Szabo C;Tulassay Z;Levine M
牙齿生物膜含有一种抑制哺乳动物细胞生长的蛋白质，可能是来自腐蚀艾肯氏菌的赖氨酸脱羧酶。这种酶使赖氨酸脱羧，赖氨酸是牙龈沟中牙齿附着细胞周转的必需氨基酸。赖氨酸耗尽可能会停止这种周转，损害细菌化合物的屏障。本研究的目的是确定生物膜赖氨酸和尸胺含量前的口腔卫生限制（OHR），并与菌斑指数（PI）和龈沟液（GCF）OHR一周后的关系。采用毛细管电泳后激光诱导荧光法测定口腔黏膜生物膜、舌生物膜和唾液中赖氨酸和尸胺的含量。在OHR之前，牙齿生物膜的赖氨酸和尸胺含量相似，比唾液或舌生物膜高10倍。OHR一周后，生物膜中尸胺含量增加，赖氨酸含量减少，与生物膜中赖氨酸脱羧酶活性增加一致。回归分析表明，PI和GCF渗出与OHR后生物膜赖氨酸呈正相关，除非生物膜赖氨酸超过最低血浆含量，在这种情况下，PI进一步增加，但GCF渗出减少。OHR后，赖氨酸脱羧酶活性似乎决定生物膜赖氨酸含量和生物膜积累。当生物膜赖氨酸超过OHR后最低血浆含量时，尽管有更多的生物膜，但GCF出现较少。赖氨酸似乎对生物膜积累和细菌促炎剂的上皮屏障很重要。抑制赖氨酸脱羧酶可以延缓微生物发育和牙龈炎所需的GCF渗出增加。
Dental biofilms contain a protein that inhibits mammalian cell growth, possibly lysine decarboxylase from Eikenella corrodens. This enzyme decarboxylates lysine, an essential amino acid for dentally attached cell turnover in gingival sulci. Lysine depletion may stop this turnover, impairing the barrier to bacterial compounds. The aims of this study were to determine biofilm lysine and cadaverine contents before oral hygiene restriction (OHR), and their association with plaque index (PI) and gingival crevicular fluid (GCF) after OHR for a week. Laser-induced fluorescence after capillary electrophoresis was used to determine lysine and cadaverine contents in dental biofilm, tongue biofilm and saliva before OHR and in dental biofilm after OHR. Before OHR, lysine and cadaverine contents of dental biofilm were similar and 10-fold greater than in saliva or tongue biofilm. After a week of OHR, the biofilm content of cadaverine increased and that of lysine decreased, consistent with greater biofilm lysine decarboxylase activity. Regression indicated that PI and GCF exudation were positively related to biofilm lysine post-OHR, unless biofilm lysine exceeded the minimal blood plasma content in which case PI was further increased but GCF exudation was reduced. After OHR, lysine decarboxylase activity seems to determine biofilm lysine content and biofilm accumulation. When biofilm lysine exceeds minimal blood plasma content after OHR, less GCF appeared despite more biofilm. Lysine appears important for biofilm accumulation and the epithelial barrier to bacterial proinflammatory agents. Inhibiting lysine decarboxylase may retard the increased GCF exudation required for microbial development and gingivitis.
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影响因子：
7.6
作者：
Barnes, V. M.;Teles, R.;Guo, L.
通讯作者：
Guo, L.
影响因子：
3.8
作者：
Dadamio, Jesica;Van Tornout, Marie;Quirynen, Marc
通讯作者：
Quirynen, Marc
影响因子：
3.8
作者：
Levine, M;Progulske-Fox, A;Pan, HQ
通讯作者：
Pan, HQ
影响因子：
3.2
作者：
Bower, JM;Mulvey, MA
通讯作者：
Mulvey, MA
影响因子：
3
作者：
LEVINE, M
通讯作者：
LEVINE, M