Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis
Roseburia intestinalis inhibits oncostatin M and maintains tight junction integrity in a murine model of acute experimental colitis
复制标题
肠罗斯氏菌在急性实验性结肠炎小鼠模型中抑制制瘤素 M 并维持紧密连接完整性
DOI：
10.1080/00365521.2019.1595708
复制
发表时间：
2019-04
影响因子：
1.9
通讯作者：
Wang Xiaoyan
中科院分区：
文献类型：
--
作者：
Tan Bei;Luo Weiwei;Shen Zhaohua;Xiao Mengwei;Wu Shuai;Meng Xiangrui;Wu Xing;Yang Zhenyu;Tian Li;Wang Xiaoyan
Abstract Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota predict responses to anti-TNF agents used for IBD therapy. Here, the aim was to investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and on intestinal barrier in colitis. Methods: In the murine model of 3% dextran sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), colon length, histological score and expression of tight junction (TJ) proteins (ZO-1, occludin and claudin-1), OSM, TNF-α and TLR5. In addition, a cellular model was used to examine the role of R. intestinalis during secretion of OSM by lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated from wild-type (WT) and TLR5 knockout (TLR5 KO) mice. Furthermore, we evaluated the impact of OSM on expressions of TJ proteins by Caco-2 cells. Results: R. intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length shortening (6.46 ± 0.36 cm vs 5.65 ± 0.47 cm, p = .022), histological score (2.667 ± 1.15 vs 5.33 ± 1.14, p = .018) and increased expression of TJ proteins (p < .05). In addition, R. intestinalis reduced expression of OSM (p < .05) and TNF-α (p < .05), while increasing expression of TLR5 (p < .05). Furthermore, R. intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated from WT and TLR5 KO mice. Moreover, OSM downregulated expression of TJ proteins (p < .05) by Caco-2 cells in a concentration-dependent manner. Conclusions: These results indicate that R. intestinalis attenuates inflammation in IBD by decreasing secretion of OSM and by promoting intestinal barrier function. Taken together, the data provide insight into the role of the gut microbiota in patients with IBD who are resistant to anti-TNF therapy.
登录
查看更多内容
影响因子：
9.8
作者：
Kolho, Kaija-Leena;Korpela, Katri;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
4.1
作者：
Hirano, Atsushi;Umeno, Junji;Esaki, Motohiro
通讯作者：
Esaki, Motohiro
影响因子：
65.1
作者：
de Souza, Heitor S. P.;Fiocchi, Claudio;Iliopoulos, Dimitrios
通讯作者：
Iliopoulos, Dimitrios
影响因子：
2.5
作者：
Takata, Fuyuko;Sumi, Noriko;Kataoka, Yasufumi
通讯作者：
Kataoka, Yasufumi
影响因子：
4.2
作者：
Simeoli, Raffaele;Raso, Giuseppina Mattace;Meli, Rosaria
通讯作者：
Meli, Rosaria