Tissue Nanotransfection Silicon Chip and Related Electroporation-Based Technologies for In Vivo Tissue Reprogramming.
Tissue Nanotransfection Silicon Chip and Related Electroporation-Based Technologies for In Vivo Tissue Reprogramming.
复制标题
用于体内组织重编程的组织纳米转染硅芯片和相关电穿孔技术。
DOI：
10.3390/nano14020217
复制
发表时间：
2024-01-19
期刊：
影响因子：
5.3
通讯作者：
Sen, Chandan K.
中科院分区：
文献类型：
--
作者：
Xuan, Yi;Wang, Cong;Ghatak, Subhadip;Sen, Chandan K.
组织纳米转染（Tissue nanotransfection， TNT）是一种体内基因治疗的前沿技术，在再生医学中的体内组织重编程、伤口愈合和癌症治疗等领域得到了广泛的应用。这项技术利用了半导体工艺的进步，促进了传统的透皮基因传递方法-纳米电穿孔和微针技术的整合。在临床前研究中，TNT硅芯片在将体内皮肤成纤维细胞重编程为血管或神经细胞，以帮助受伤肢体和受损脑组织的恢复方面显示出相当大的前景。最近，TNT芯片的应用已经扩展到外泌体领域，外泌体对于细胞内通信至关重要，可以在伤口愈合过程中跟踪其功能。在这篇综述中，我们对TNT硅芯片的设计、制造和应用进行了深入的研究，同时对基于电穿孔的基因转移机制进行了批判性的分析。此外，本文还讨论了当前技术存在的局限性和挑战，这可能会预测基因治疗未来的发展轨迹。通过这一探索，综述旨在阐明TNT在基因治疗和组织再生的更广泛背景下的前景。
Tissue nanotransfection (TNT), a cutting-edge technique of in vivo gene therapy, has gained substantial attention in various applications ranging from in vivo tissue reprogramming in regenerative medicine, and wound healing to cancer treatment. This technique harnesses the advancements in the semiconductor processes, facilitating the integration of conventional transdermal gene delivery methods—nanoelectroporation and microneedle technologies. TNT silicon chips have demonstrated considerable promise in reprogramming fibroblast cells of skin in vivo into vascular or neural cells in preclinical studies to assist in the recovery of injured limbs and damaged brain tissue. More recently, the application of TNT chips has been extended to the area of exosomes, which are vital for intracellular communication to track their functionality during the wound healing process. In this review, we provide an in-depth examination of the design, fabrication, and applications of TNT silicon chips, alongside a critical analysis of the electroporation-based gene transfer mechanisms. Additionally, the review discussed the existing limitations and challenges in the current technique, which may project future trajectories in the landscape of gene therapy. Through this exploration, the review aims to shed light on the prospects of TNT in the broader context of gene therapy and tissue regeneration.
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影响因子：
3.9
作者：
Corovic S;Lackovic I;Sustaric P;Sustar T;Rodic T;Miklavcic D
通讯作者：
Miklavcic D
影响因子：
38.3
作者：
Gallego-Perez D;Pal D;Ghatak S;Malkoc V;Higuita-Castro N;Gnyawali S;Chang L;Liao WC;Shi J;Sinha M;Singh K;Steen E;Sunyecz A;Stewart R;Moore J;Ziebro T;Northcutt RG;Homsy M;Bertani P;Lu W;Roy S;Khanna S;Rink C;Sundaresan VB;Otero JJ;Lee LJ;Sen CK
通讯作者：
Sen CK
DOI：
10.3390/bios11090296
发表时间：
2021-08-25
期刊：
Biosensors
影响因子：
--
作者：
Erdem Ö;Eş I;Akceoglu GA;Saylan Y;Inci F
通讯作者：
Inci F
影响因子：
7.4
作者：
Brown, Brooke A.;Guda, Poornachander R.;Zeng, Xuyao;Anthony, Adam;Couse, Andrew;Barnes, Lauren F.;Sharon, Edie M.;Trinidad, Jonathan C.;Sen, Chandan K.;Jarrold, Martin F.;Ghatak, Subhadip;Clemmer, David E.
通讯作者：
Clemmer, David E.
影响因子：
3.9
作者：
Badnikar, Kedar;Jayadevi, Shreyas N.;Subrahmanyam, Dinesh N.
通讯作者：
Subrahmanyam, Dinesh N.