IRESite--a tool for the examination of viral and cellular internal ribosome entry sites.

IRESite--a tool for the examination of viral and cellular internal ribosome entry sites.
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DOI:
10.1093/nar/gkp981
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发表时间:
2010-01
影响因子:
14.9
通讯作者:
Pospísek M
Pospísek M
中科院分区:
生物学2区
文献类型:
--
作者:
Mokrejs M;Masek T;Vopálensky V;Hlubucek P;Delbos P;Pospísek M

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IRESite(www.iresite.org)提供了许多真核病毒和细胞内部核糖体进入位点(IRES)区域的精心策划的实验证据。在提交时,IRESite储存了超过600条记录。IRESite逐渐发展成为一个强大的工具,提供(i)关于IRES及其实验背景的生物学意义信息(包括IRES二级结构和IRES反式作用因子的注释)以及(ii)对存储的数据库条目的全面总结性评论和定期更新报告的IRES功能的评估。IRESite数据的很大一部分纯粹来自于对现有序列的内部生物信息学分析,通过计算机模拟尝试重复已发表的克隆实验,DNA测序和对收到的质粒DNA的限制性内切酶验证。我们还提出了一个新实现的工具,用于显示RNA二级结构和搜索目前存储在数据库中的结构。补充材料载有已报告的可再生能源系统的最新清单。
The IRESite (http://www.iresite.org) presents carefully curated experimental evidence of many eukaryotic viral and cellular internal ribosome entry site (IRES) regions. At the time of submission, IRESite stored >600 records. The IRESite gradually evolved into a robust tool providing (i) biologically meaningful information regarding the IRESs and their experimental background (including annotation of IRES secondary structures and IRES trans-acting factors) as well as (ii) thorough concluding remarks to stored database entries and regularly updated evaluation of the reported IRES function. A substantial portion of the IRESite data results purely from in-house bioinformatic analyses of currently available sequences, in silico attempts to repeat published cloning experiments, DNA sequencing and restriction endonuclease verification of received plasmid DNA. We also present a newly implemented tool for displaying RNA secondary structures and for searching through the structures currently stored in the database. The supplementary material contains an updated list of reported IRESs.
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发表时间: 2009-08-01
期刊: Bioinformatics (Oxford, England)
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