Characterization of metabolic states of Arabidopsis thaliana under diverse carbon and nitrogen nutrient conditions via targeted metabolomic analysis.
Characterization of metabolic states of Arabidopsis thaliana under diverse carbon and nitrogen nutrient conditions via targeted metabolomic analysis.
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DOI：
10.1093/pcp/pct192
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发表时间：
2014-02
影响因子：
4.9
通讯作者：
Yanagisawa S
中科院分区：
文献类型：
--
作者：
Sato S;Yanagisawa S
植物的生长和代谢受各种环境因素的调节。为了研究大气CO2浓度升高和其他营养因素的联合作用对植物代谢的调节作用，我们使用在24种不同条件下生长的拟南芥植物进行了有针对性的代谢组学分析，其中CO2浓度，氮源的数量和种类以及光照强度进行了修改。我们的研究结果表明，在广泛的CO2水平下，从环境浓度到极高浓度（3，600 p.p.m.），不同代谢物的生物合成和生长都与CO2浓度成比例地促进。二氧化碳。这表明A.拟南芥具有有效利用非常高浓度的CO2的潜力。另一方面，铵（而不是硝酸盐）作为额外的氮源供应诱导代谢物组成的急剧变化，包括葡萄糖，淀粉和几种氨基酸的含量增加，并减少在三羧酸（TCA）循环相关的有机酸含量在任何CO2条件下。使用代谢产物谱的层次聚类分析显示，铵是一个突出的因素，而CO2浓度不是决定代谢状态。然而，铵诱导的代谢改变不同的修改高浓度的CO2。因此，我们的研究结果意味着，CO2浓度的增加可能会不同地影响植物的代谢，这取决于氮营养条件。
Plant growth and metabolism are regulated in response to various environmental factors. To investigate modulations in plant metabolism by the combined action of elevated atmospheric CO2 concentration and other nutritional factors, we performed targeted metabolomic analysis using Arabidopsis thaliana plants grown under 24 different conditions where the CO2 concentration, amounts and species of nitrogen source, and light intensity were modified. Our results indicate that both the biosynthesis of diverse metabolites and growth are promoted in proportion to the CO2 concentration at a wide range of CO2 levels, from ambient concentrations to an extremely high concentration (3,600 p.p.m.) of CO2. This suggests that A. thaliana has the potential to utilize effectively very high concentrations of CO2. On the other hand, ammonium (but not nitrate) supplied as an additional nitrogen source induced drastic alterations in metabolite composition, including increases in the contents of glucose, starch and several amino acids, and reductions in the tricarboxylic acid (TCA) cycle-related organic acid content under any CO2 conditions. Hierarchical clustering analysis using the metabolite profiles revealed that ammonium is a prominent factor determining metabolic status, while the CO2 concentration is not. However, ammonium-induced metabolic alterations were differently modified by high concentrations of CO2. Hence, our results imply that increases in CO2 concentration may differently influence plant metabolism depending on the nitrogen nutrient conditions.
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影响因子：
7.4
作者：
SAGE, RF;SHARKEY, TD;SEEMANN, JR
通讯作者：
SEEMANN, JR
DOI：
10.1111/j.1365-313x.2011.04848.x
发表时间：
2012-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Engelsberger WR;Schulze WX
通讯作者：
Schulze WX
影响因子：
6.9
作者：
Lawlor, DW
通讯作者：
Lawlor, DW
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
6.9
作者：
Foyer, CH;Parry, M;Noctor, G
通讯作者：
Noctor, G