Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by Toxoplasma gondii.
Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by Toxoplasma gondii.
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DOI：
10.3390/molecules27175572
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发表时间：
2022-08-30
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影响因子：
--
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中科院分区：
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背景资料：弓形虫是一种寄生于宿主脑内的胞内原虫，以包囊形式存在于宿主脑内，可引起弓形虫脑炎和神经系统疾病。以往的蛋白质组学研究表明，T.弓形虫感染方法：本研究采用T.弓形虫感染小鼠和bEnd3细胞，以证实弓形虫感染小鼠和bEnd3细胞之间的关系。C3.通过pull down筛选与C3a直接相互作用的BEnd3细胞膜蛋白。最后，进行动物行为学实验以比较四种化疗化合物（SB 290157、CVF、NSC23766和Anxa 1）对TE的抑制能力的差异。结果：本研究中的所有化疗化合物均能抑制TE和宿主的认知行为。而Anxa 1是抑制小鼠TE的最佳材料。结论：T.弓形虫感染通过促进宿主C3的产生而促进TE。在与C3密切相关的四种化疗化合物中，Anxa1被选为预防TE的最合适的材料。
Background: Toxoplasma gondii, an intracellular protozoan parasite, exists in the host brain as cysts, which can result in Toxoplasmic Encephalitis (TE) and neurological diseases. However, few studies have been conducted on TE, particularly on how to prevent it. Previous proteomics studies have showed that the expression of C3 in rat brains was up-regulated after T. gondii infection. Methods: In this study, we used T. gondii to infect mice and bEnd 3 cells to confirm the relation between T. gondii and the expression of C3. BEnd3 cells membrane proteins which directly interacted with C3a were screened by pull down. Finally, animal behavior experiments were conducted to compare the differences in the inhibitory ability of TE by four chemotherapeutic compounds (SB290157, CVF, NSC23766, and Anxa1). Results: All chemotherapeutic compounds in this study can inhibit TE and cognitive behavior in the host. However, Anxa 1 is the most suitable material to inhibit mice TE. Conclusion: T. gondii infection promotes TE by promoting host C3 production. Anxa1 was selected as the most appropriate material to prevent TE among four chemotherapeutic compounds closely related to C3.
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