The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro.
The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro.
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DOI：
10.1186/1471-2180-14-133
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发表时间：
2014-05-22
期刊：
影响因子：
4.2
通讯作者：
Van de Wiele T
中科院分区：
文献类型：
--
作者：
Marzorati M;Vanhoecke B;De Ryck T;Sadaghian Sadabad M;Pinheiro I;Possemiers S;Van den Abbeele P;Derycke L;Bracke M;Pieters J;Hennebel T;Harmsen HJ;Verstraete W;Van de Wiele T
最近的科学发展已经揭示了宿主-微生物相互作用的重要性，特别是在肠道中。然而，宿主-微生物相互作用的机制研究由于到达体内胃肠道（GIT）的不同区域的内在限制而变得复杂。在本文中，我们提出了一种新设备的技术验证-主机-微生物群相互作用（HMI）模块-和证据，它可以与肠道动态模拟器结合使用，以评估特定治疗的效果在管腔微生物群落和主机表面定植和信号的水平。HMI模块重新创建与GIT生理相关的条件：i）在相关剪切应力下细菌可以粘附的粘膜区域（3达因cm−2）; ii）低分子量代谢物的双侧转运（4至150 kDa），渗透系数范围为2.4 × 10−6至7.1 × 10−9 cm sec−1;和iii）生长生物膜底部的微需氧条件（PmO 2 = 2.5 × 10 - 4 cm sec-1）。在一项长期研究中，HMI模块中的宿主细胞在暴露于复杂的微生物群落48小时后仍然存活。占主导地位的粘液相关的微生物群不同于管腔的，其组成受到来自酵母发酵的干燥产品的治疗。后者-具有已知的抗炎特性-在24和48小时之间诱导促炎性IL-8产生的减少。在人体GIT中粘附细菌群落的体内功能性和对宿主的局部作用的研究经常受到到达GIT特定区域的复杂性的阻碍。HMI模块提供了将肠道代表性微生物群落与肠细胞样细胞共培养长达48小时的可能性，因此可能有助于对宿主-微生物组相互作用的机制理解。
Recent scientific developments have shed more light on the importance of the host-microbe interaction, particularly in the gut. However, the mechanistic study of the host-microbe interplay is complicated by the intrinsic limitations in reaching the different areas of the gastrointestinal tract (GIT) in vivo. In this paper, we present the technical validation of a new device - the Host-Microbiota Interaction (HMI) module - and the evidence that it can be used in combination with a gut dynamic simulator to evaluate the effect of a specific treatment at the level of the luminal microbial community and of the host surface colonization and signaling. The HMI module recreates conditions that are physiologically relevant for the GIT: i) a mucosal area to which bacteria can adhere under relevant shear stress (3 dynes cm−2); ii) the bilateral transport of low molecular weight metabolites (4 to 150 kDa) with permeation coefficients ranging from 2.4 × 10−6 to 7.1 × 10−9 cm sec−1; and iii) microaerophilic conditions at the bottom of the growing biofilm (PmO2 = 2.5 × 10−4 cm sec−1). In a long-term study, the host’s cells in the HMI module were still viable after a 48-hour exposure to a complex microbial community. The dominant mucus-associated microbiota differed from the luminal one and its composition was influenced by the treatment with a dried product derived from yeast fermentation. The latter - with known anti-inflammatory properties - induced a decrease of pro-inflammatory IL-8 production between 24 and 48 h. The study of the in vivo functionality of adhering bacterial communities in the human GIT and of the localized effect on the host is frequently hindered by the complexity of reaching particular areas of the GIT. The HMI module offers the possibility of co-culturing a gut representative microbial community with enterocyte-like cells up to 48 h and may therefore contribute to the mechanistic understanding of host-microbiome interactions.
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影响因子：
4.9
作者：
Lepage, P;Seksik, P;Doré, J
通讯作者：
Doré, J
影响因子：
4
作者：
Li, X. J.;Yue, L. Y.;Qiao, S. Y.
通讯作者：
Qiao, S. Y.
DOI：
10.1073/pnas.0908876106
发表时间：
2009-10-06
影响因子：
11.1
作者：
Kankainen, Matti;Paulin, Lars;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
DOI：
10.1016/j.ijfoodmicro.2010.09.007
发表时间：
2010-11-15
影响因子：
5.4
作者：
Grimoud, Julien;Durand, Henri;Rogues, Christine
通讯作者：
Rogues, Christine
影响因子：
4.4
作者：
Boon, N;Top, EM;Siciliano, SD
通讯作者：
Siciliano, SD