A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.
A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.
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DOI:
10.1002/1873-3468.13249
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发表时间:
2018-10
期刊:
影响因子:
3.5
通讯作者:
Assmann SM
中科院分区:
文献类型:
--
作者:
Chakravorty D;Yu Y;Assmann SM
The receptor-like kinase FERONIA (FER) pleiotropically affects plant reproduction, development, and stress tolerance. We recently showed that the FER ligand RALF1 promotes stomatal closure and inhibits stomatal opening in a G-protein-dependent manner. FER responses have been designated as kinase-dependent or kinase-independent, based largely on fer complementation assays employing a kinase-dead FERK565R. Our quantification of FERK565R-GFP transcript and FERK565R-GFP protein in fer complementation lines reveal that, even within individual complementation lines, different levels of FERK565R expression prevail. FERK565R-GFP expression comparable to that of FER in Col-0 plants fail to elicit complementation of either fer rosette phenotypes or RALF1-elicited stomatal movements, while overexpression levels of FERK565R-GFP result in complementation. These results suggest possible alternative interpretations of previous conclusions regarding kinase-independent FER signaling. Lines of a feronia (fer) null mutant transformed with a construct encoding kinase-dead FER show within-line and between-line dose-dependent complementation of rosette morphology and RALF1-mediated stomatal responses.
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DOI:
10.1126/science.1244454
发表时间:
2014-01-24
期刊:
Science (New York, N.Y.)
影响因子:
--
作者:
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通讯作者:
Sussman MR
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DOI:
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发表时间:
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影响因子:
11.1
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影响因子:
7.4
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DOI:
10.1073/pnas.1608449113
发表时间:
2016-09-13
影响因子:
11.1
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