A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.

A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.
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DOI:
10.1002/1873-3468.13249
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发表时间:
2018-10
期刊:
影响因子:
3.5
通讯作者:
Assmann SM
Assmann SM
中科院分区:
生物学3区
文献类型:
--
作者:
Chakravorty D;Yu Y;Assmann SM

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受体样激酶 FERONIA (FER) 多效性影响植物繁殖、发育和胁迫耐受性。我们最近表明,FER 配体 RALF1 以 G 蛋白依赖性方式促进气孔关闭并抑制气孔打开。 FER 反应已被指定为激酶依赖性或激酶非依赖性,主要基于使用激酶死亡 FERK565R 的 fer 互补测定。我们对 fer 互补系中 FERK565R-GFP 转录物和 FERK565R-GFP 蛋白的定量表明,即使在单个互补系内,也存在不同水平的 FERK565R 表达。 FERK565R-GFP 表达与 Col-0 植物中的 FER 相当,无法引起 fer 莲座表型或 RALF1 引起的气孔运动的互补,而 FERK565R-GFP 的过表达水平会导致互补。这些结果表明了对先前关于不依赖于激酶的 FER 信号传导的结论的可能的替代解释。用编码激酶死亡 FER 的构建体转化的 feronia (fer) 无效突变体的品系显示出株内和株间玫瑰花结形态和 RALF1 介导的气孔反应的剂量依赖性互补。
The receptor-like kinase FERONIA (FER) pleiotropically affects plant reproduction, development, and stress tolerance. We recently showed that the FER ligand RALF1 promotes stomatal closure and inhibits stomatal opening in a G-protein-dependent manner. FER responses have been designated as kinase-dependent or kinase-independent, based largely on fer complementation assays employing a kinase-dead FERK565R. Our quantification of FERK565R-GFP transcript and FERK565R-GFP protein in fer complementation lines reveal that, even within individual complementation lines, different levels of FERK565R expression prevail. FERK565R-GFP expression comparable to that of FER in Col-0 plants fail to elicit complementation of either fer rosette phenotypes or RALF1-elicited stomatal movements, while overexpression levels of FERK565R-GFP result in complementation. These results suggest possible alternative interpretations of previous conclusions regarding kinase-independent FER signaling. Lines of a feronia (fer) null mutant transformed with a construct encoding kinase-dead FER show within-line and between-line dose-dependent complementation of rosette morphology and RALF1-mediated stomatal responses.
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