Development of sensor cells using NF-κB pathway activation for detection of nanoparticle-induced inflammation.
Development of sensor cells using NF-κB pathway activation for detection of nanoparticle-induced inflammation.
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DOI：
10.3390/s110707219
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Taniguchi A
中科院分区：
文献类型：
--
作者：
Chen P;Migita S;Kanehira K;Sonezaki S;Taniguchi A
纳米材料在消费品和工业产品中的使用日益增加，引起了人们对其在生物系统中的命运的关注。因此，一种有效的检测方法来评价生物纳米材料的安全性是非常重要的。二氧化钛（TiO2）在世界范围内大量生产，用于广泛的应用，包括颜料和化妆品制造。二氧化钛曾被认为是一种惰性材料，但最近，越来越多的研究表明，二氧化钛纳米粒子（TiO2 NPs）可以引起炎症，并通过引起肺部和脑部问题对人体有害。为了评估TiO2 NPs对环境和人体的安全性，我们开发了用于炎症检测的传感细胞，并转染了toll样受体4 （TLR4）基因和核因子κB （NF-κB）报告基因。NF-κB作为炎症的主要原因受到了广泛的关注，它可以被多种外部刺激激活。我们的数据表明，TiO2 nps诱导的炎症可以被我们的传感器细胞通过NF-κB通路激活来检测。这可能会导致我们的传感器细胞被用于生物纳米材料的安全性评估。
The increasing use of nanomaterials in consumer and industrial products has aroused concerns regarding their fate in biological systems. An effective detection method to evaluate the safety of bio-nanomaterials is therefore very important. Titanium dioxide (TiO2), which is manufactured worldwide in large quantities for use in a wide range of applications, including pigment and cosmetic manufacturing, was once thought to be an inert material, but recently, more and more studies have indicated that TiO2 nanoparticles (TiO2 NPs) can cause inflammation and be harmful to humans by causing lung and brain problems. In order to evaluate the safety of TiO2 NPs for the environment and for humans, sensor cells for inflammation detection were developed, and these were transfected with the Toll-like receptor 4 (TLR4) gene and Nuclear Factor Kappa B (NF-κB) reporter gene. NF-κB as a primary cause of inflammation has received a lot of attention, and it can be activated by a wide variety of external stimuli. Our data show that TiO2 NPs-induced inflammation can be detected by our sensor cells through NF-κB pathway activation. This may lead to our sensor cells being used for bio-nanomaterial safety evaluation.
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