Culture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris.
Culture Degeneration Reduces Sex-Related Gene Expression, Alters Metabolite Production and Reduces Insect Pathogenic Response in Cordyceps militaris.
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DOI：
10.3390/microorganisms9081559
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发表时间：
2021-07-22
期刊：
影响因子：
4.5
通讯作者：
de Moor CH
中科院分区：
文献类型：
--
作者：
Wellham PAD;Hafeez A;Gregori A;Brock M;Kim DH;Chandler D;de Moor CH
蛹虫草（Cordycepsmilitaris）是一种昆虫病原性子囊菌，主要以感染鳞翅目幼虫（毛虫）和蛹宿主而闻名。虫草素是虫草产生的一种次级代谢产物，具有抗炎等药理活性。然而，很少有人知道这种腺苷衍生物及其稳定化合物喷司他丁在昆虫感染的情况下的生物学作用。军队。在实验室条件下反复继代培养，C.可发生以虫草素生产水平降低为特征的蛹虫草。在这里，使用退化和亲本对照菌株的分离的C。在蛹虫草中，我们发现虫草素产量的降低与各种其他代谢物产量的下降以及与性发育相关的基因表达的减少相一致。此外，感染大蜡螟幼虫表明虫草素抑制宿主血细胞的免疫反应。因此，对退化菌株的致病性应答降低。这些数据表明，在有性生殖、次级代谢产物的产生、昆虫免疫和C.军队。本研究对利用真菌防治作物害虫具有一定的指导意义。
Cordycepsmilitaris is an entomopathogenic ascomycete, known primarily for infecting lepidopteran larval (caterpillars) and pupal hosts. Cordycepin, a secondary metabolite produced by this fungus has anti-inflammatory properties and other pharmacological activities. However, little is known about the biological role of this adenosine derivate and its stabilising compound pentostatin in the context of insect infection the life cycle of C. militaris. During repeated subcultivation under laboratory conditions a degeneration of C. militaris marked by decreasing levels of cordycepin production can occur. Here, using degenerated and parental control strains of an isolate of C. militaris, we found that lower cordycepin production coincides with the decline in the production of various other metabolites as well as the reduced expression of genes related to sexual development. Additionally, infection of Galleria mellonella (greater wax moth) caterpillars indicated that cordycepin inhibits the immune response in host haemocytes. Accordingly, the pathogenic response to the degenerated strain was reduced. These data indicate that there are simultaneous changes in sexual reproduction, secondary metabolite production, insect immunity and infection by C. militaris. This study may have implications for biological control of insect crop pests by fungi.
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