Entrapment of probiotics in water extractable arabinoxylan gels: rheological and microstructural characterization.
Entrapment of probiotics in water extractable arabinoxylan gels: rheological and microstructural characterization.
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DOI：
10.3390/molecules19033628
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发表时间：
2014-03-24
期刊：
影响因子：
--
通讯作者：
Campa-Mada AC
中科院分区：
文献类型：
--
作者：
Morales-Ortega A;Carvajal-Millan E;Brown-Bojorquez F;Rascón-Chu A;Torres-Chavez P;López-Franco YL;Lizardi-Mendoza J;Martínez-López AL;Campa-Mada AC
由于其多孔结构，水环境和膳食纤维天然阿拉伯木聚糖（AX）凝胶在结肠特异性治疗分子递送方面具有潜在的应用前景。此外，AX的益生元和健康相关作用先前已得到证实。也有报道称，交联的AX可以被肠道微生物群中的细菌降解。然而，AX凝胶作为载体系统还没有被大量研究，也没有关于其诱捕细胞能力的信息。在这方面，长双歧杆菌等益生菌近年来一直是研究的热点。本研究的目的是研究益生菌长芽孢杆菌在AX凝胶中的包裹情况。以漆酶为交联剂，在2% （w/v）浓度的AX溶液中，含有1 × 107 CFU/cm的长芽孢杆菌形成凝胶。长芽孢杆菌的滞留使凝胶弹性从31 Pa降低到23 Pa，这可能是由于影响了WEAX链之间的物理相互作用。在扫描电子显微镜下观察含有长芽孢杆菌的AX凝胶的图像，可以看到凝胶网络中包裹着细菌细胞。这些凝胶的微观结构类似于不完美的蜂巢。结果表明，AX凝胶可以作为益生菌包埋的潜在候选物。
Due to their porous structure, aqueous environment and dietary fiber nature arabinoxylan (AX) gels could have potential applications for colon-specific therapeutic molecule delivery. In addition, prebiotic and health related effects of AX have been previously demonstrated. It has been also reported that cross-linked AX can be degraded by bacteria from the intestinal microbiota. However, AX gels have not been abundantly studied as carrier systems and there is no information available concerning their capability to entrap cells. In this regard, probiotic bacteria such as Bifidobacterium longum have been the focus of intense research activity lately. The objective of this research was to investigate the entrapment of probiotic B. longum in AX gels. AX solution at 2% (w/v) containing B. longum (1 × 107 CFU/cm) formed gels induced by laccase as cross-linking agent. The entrapment of B. longum decreased gel elasticity from 31 to 23 Pa, probably by affecting the physical interactions taking place between WEAX chains. Images of AX gels containing B. longum viewed under a scanning electron microscope show the gel network with the bacterial cells entrapped inside. The microstructure of these gels resembles that of an imperfect honeycomb. The results suggest that AX gels can be potential candidates for the entrapment of probiotics.
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影响因子：
5.4
作者：
Gbassi GK;Vandamme T
通讯作者：
Vandamme T
影响因子：
4.4
作者：
Hopkins, MJ;Englyst, HN;McBain, AJ
通讯作者：
McBain, AJ
影响因子：
5.5
作者：
Zhu, SM;Ramaswamy, HS;Le Bail, A
通讯作者：
Le Bail, A
影响因子：
4
作者：
Silva, AM;Barbosa, FHF;Nicoli, JR
通讯作者：
Nicoli, JR
Arabinoxylan微球：结构和纹理特征。
DOI：
10.3390/molecules18044640
发表时间：
2013-04-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Martínez-López AL;Carvajal-Millan E;Miki-Yoshida M;Alvarez-Contreras L;Rascón-Chu A;Lizardi-Mendoza J;López-Franco Y
通讯作者：
López-Franco Y