Characterization of Endophytic Fungi, Acremonium sp., from Lilium davidii and Analysis of Its Antifungal and Plant Growth-Promoting Effects.
Characterization of Endophytic Fungi, Acremonium sp., from Lilium davidii and Analysis of Its Antifungal and Plant Growth-Promoting Effects.
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DOI：
10.1155/2021/9930210
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发表时间：
2021
影响因子：
--
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Khan MS;Gao J;Munir I;Zhang M;Liu Y;Moe TS;Xue J;Zhang X
本研究旨在从亚洲食药植物百合中分离内生真菌，并分析其抗真菌和促进植物生长的作用。本研究从大灰莲球茎中分离到一株内生真菌Acremonium sp. Ld-03，并通过形态和分子分析对其进行鉴定。分子和形态分析证实该内生真菌菌株为Acremonium sp. Ld-03。观察了Ld-03对尖孢镰刀菌、灰霉病菌、斑点孢镰刀菌和藤黑镰刀菌的抑菌效果。结果表明，该菌的生长抑制率最高，为78.39±4.21%，其次为灰灰双霉病菌（56.68±4.38%）、藤黑双霉病菌（43.62±3.81%）和尖孢双霉病菌（20.12±2.45%）。通过UHPLC-LTQ-IT-MS/MS对乙酸乙酯部分进行分析，发现其次生代谢产物包括黄嘌呤酸、戊酰天冬氨酸、甘酸苷W、多肽和环二肽，如缬氨酸、环-[L-(4-羟基- pro)-L-leu]、环（Pro-Phe）和(3S,6S)-3-苄基-6-（4-羟基苄基）哌嗪-2,5-二酮。其他代谢物包括（S)-3-（4-羟基苯基）-2-(S)-吡啶-2-羧胺）丙酸、邻苯二甲酸二丁酯（DBP）、9-十八烯酰胺、d -红- c18 -鞘氨醇、n -棕榈酰鞘氨酰和羟棕榈酰鞘氨酰。菌株Ld-03在添加或不添加外源色氨酸的情况下均能产生吲哚乙酸（IAA）。不同色氨酸浓度下的IAA范围为53.12±3.20 ~ 167.71±7.12 μ ml−1。菌株能够产生铁载体，其产量随着培养基中铁（III）柠檬酸盐浓度的增加而显著降低。该内生真菌菌株还表现出有机酸的生产和磷酸盐的增溶活性。研究了该菌株对大葱离体苗生长的促进作用。与未处理的对照植株相比，稀释40%后的根长和茎长分别增加了24.03±2.71 mm和37.27±1.86 mm。内生真菌Ld-03具有抗病和促进植物生长的潜力。综上所述，在将分离得到的顶孢杆菌（Acremonium sp. Ld-03）作为一种生物防治剂和植物生长刺激剂加以利用之前，还需进一步研究。
The present study was aimed at isolating endophytic fungi from the Asian culinary and medicinal plant Lilium davidii and analyzing its antifungal and plant growth-promoting effects. In this study, the fungal endophyte Acremonium sp. Ld-03 was isolated from the bulbs of L. davidii and identified through morphological and molecular analysis. The molecular and morphological analysis confirmed the endophytic fungal strain as Acremonium sp. Ld-03. Antifungal effects of Ld-03 were observed against Fusarium oxysporum, Botrytis cinerea, Botryosphaeria dothidea, and Fusarium fujikuroi. The highest growth inhibition, i.e., 78.39 ± 4.21%, was observed for B. dothidea followed by 56.68 ± 4.38%, 43.62 ± 3.81%, and 20.12 ± 2.45% for B. cinerea, F. fujikuroi, and F. oxysporum, respectively. Analysis of the ethyl acetate fraction through UHPLC-LTQ-IT-MS/MS revealed putative secondary metabolites which included xanthurenic acid, valyl aspartic acid, gancidin W, peptides, and cyclic dipeptides such as valylarginine, cyclo-[L-(4-hydroxy-Pro)-L-leu], cyclo(Pro-Phe), and (3S,6S)-3-benzyl-6-(4-hydroxybenzyl)piperazine-2,5-dione. Other metabolites included (S)-3-(4-hydroxyphenyl)-2-((S)-pyrrolidine-2-carboxamido)propanoic acid, dibutyl phthalate (DBP), 9-octadecenamide, D-erythro-C18-Sphingosine, N-palmitoyl sphinganine, and hydroxypalmitoyl sphinganine. The strain Ld-03 showed indole acetic acid (IAA) production with or without the application of exogenous tryptophan. The IAA ranged from 53.12 ± 3.20 μg ml−1 to 167.71 ± 7.12 μg ml−1 under different tryptophan concentrations. The strain was able to produce siderophore, and its production was significantly decreased with increasing Fe(III) citrate concentrations in the medium. The endophytic fungal strain also showed production of organic acids and phosphate solubilization activity. Plant growth-promoting effects of the strain were evaluated on in vitro seedling growth of Allium tuberosum. Application of 40% culture dilution resulted in a significant increase in root and shoot length, i.e., 24.03 ± 2.71 mm and 37.27 ± 1.86 mm, respectively, compared to nontreated control plants. The fungal endophyte Ld-03 demonstrated the potential of conferring disease resistance and plant growth promotion. Therefore, we conclude that the isolated Acremonium sp. Ld-03 should be further investigated before utilization as a biocontrol agent and plant growth stimulator.
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影响因子：
3.4
作者：
Cao, Ying;Fu, Dafang;Hu, Zhixin
通讯作者：
Hu, Zhixin
影响因子：
20.3
作者：
Hamilton, Cyd E.;Bauerle, Taryn L.
通讯作者：
Bauerle, Taryn L.
影响因子：
2.8
作者：
Fang, Xu;Yano, Shinichi;Sawayama, Shigeki
通讯作者：
Sawayama, Shigeki
影响因子：
4.4
作者：
CUNNINGHAM, JE;KUIACK, C
通讯作者：
KUIACK, C
DOI：
10.1080/03235408.2018.1440707
发表时间：
2018-01-01
影响因子：
1
作者：
Chowdhary, Kanika;Kaushik, Nutan
通讯作者：
Kaushik, Nutan