Mitochondrial DNA content and lung cancer risk in Xuan Wei, China.
Mitochondrial DNA content and lung cancer risk in Xuan Wei, China.
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DOI：
10.1016/j.lungcan.2008.06.012
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发表时间：
2009-03
期刊：
影响因子：
5.3
通讯作者：
Lan, Qing
中科院分区：
文献类型：
--
作者：
Bonner, Matthew R.;Shen, Min;Liu, Chin-San;DiVita, Margaret;He, Xingzhou;Lan, Qing
关键词：
烟煤含有多环芳烃（PAHs），并且在中国宣威与肺癌病因密切相关。虽然已证实多环芳烃会在核DNA中形成大的加合物，但它们对线粒体DNA（mtDNA）的亲和力要高90倍。为了补偿线粒体功能障碍或损伤，人们认为mtDNA含量会增加。我们在中国宣威进行了一项基于人群的肺癌病例对照研究，假设mtDNA含量与肺癌风险相关。病例组（n = 122）和对照组（n = 121）在年龄（±2岁）、性别、居住村庄以及当前使用的取暖/烹饪燃料类型上进行了个体匹配。通过问卷调查确定了一生使用烟煤的情况和潜在的混杂因素。从痰液中提取mtDNA，并通过定量逆转录聚合酶链反应测定其含量。通过非条件逻辑回归计算比值比（OR）和95%置信区间（95%CI）。根据对照组中的分布情况，将mtDNA含量以中位数进行二分。与mtDNA含量≤157拷贝的人相比，mtDNA含量>157的人患肺癌的风险增加了2倍（OR = 1.8；95%CI = 1.0 - 3.2）。57岁以上的人相比57岁及以下的人风险更高（交互作用p值 = 0.01）。总之，mtDNA含量与肺癌风险呈正相关。此外，有一些证据表明，在老年人中mtDNA含量与肺癌风险的相关性更强。然而，由于样本量较小，需要进一步的研究来评估这些相关性。
Smoky coal contains polycyclic aromatic hydrocarbons (PAHs) and has been strongly implicated in etiology of lung cancer in Xuan Wei, China. While PAHs have been demonstrated to form bulky adducts in nuclear DNA, they have a 90-fold greater affinity for mitochondrial DNA (mtDNA). To compensate for mitochondrial dysfunction or damage, mtDNA content is thought to increase. We conducted a population-based case-control study of lung cancer in Xuan Wei, China hypothesizing that mtDNA content is associated with lung cancer risk. Cases (n = 122) and controls (n = 121) were individually matched on age (±2yrs), sex, village of residence, and type of heating/cooking fuel currently used. Lifetime smoky coal use and potential confounders were determined with questionnaires. mtDNA was extracted from sputum and content was determined with quantitative RT-PCR. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated with unconditional logistic regression. mtDNA content was dichotomized at the median based on the distribution among the controls. mtDNA content > 157 was associated with a 2-fold increase in lung cancer risk (OR = 1.8; 95% CI = 1.0–3.2) compared with those with ≤157 copies. Risk was higher among those >57 years of age compared with those ≤ 57 years (p interaction = 0.01). In summary, mtDNA content was positively associated with lung cancer risk. Furthermore, there was some evidence that mtDNA content was more strongly associated with lung cancer risk among older individuals. However, due to the small sample size, additional studies are needed to evaluate these associations.
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影响因子：
11.5
作者：
Jiang, WW;Rosenbaum, E;Califano, JA
通讯作者：
Califano, JA
影响因子：
5.3
作者：
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通讯作者：
Meissner, C
DOI：
10.1080/00039896.1988.9935850
发表时间：
1988-03-01
期刊：
ARCHIVES OF ENVIRONMENTAL HEALTH
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
Wei, YH