Identification of novel loci affecting entry of Salmonella enteritidis into eukaryotic cells-猫眼课题宝

影响肠炎沙门氏菌进入真核细胞的新位点的鉴定

DOI：

10.1128/jb.174.12.3945-3952.1992

发表时间：

1992

期刊：

Journal of Bacteriology

影响因子：

3.2

通讯作者：

中科院分区：

生物学3区

文献类型：

作者：

B J Stone;C M Garcia;J. L. Badger;T. Hassett;R I Smith;V. L. Miller

据估计，美国每年有200万例沙门氏菌病病例。与许多传染病的发病率不同，沙门氏菌病在美国等发达国家的发病率在过去30年里一直在稳步上升，目前该疾病占美国所有急性胃肠炎病例的10%至15%。感染的微生物被吞噬，必须穿过肠道上皮，才能到达其繁殖的首选位置，即网状内皮系统。尽管最近有几项研究，但对入侵过程的遗传基础了解甚少。这些研究的一个新主题是，野生型沙门氏菌生物可能有几个染色体位点，这是最有效的入侵水平所必需的。在这项研究中，我们鉴定和鉴定了13个肠炎沙门氏菌CDC5的TnPhoA插入突变株，这些突变株的侵袭表型发生了变化。通过筛选肠炎链霉菌CDC5str中的一组突变体在三种组织培养细胞系(Hep-2、CHO和MDCK)中的侵袭表型来鉴定突变体。根据这13个突变体在组织培养细胞系中的侵袭表型，将其分为6类。两个突变体(SM6和SM7)具有进入所有三种组织培养细胞系的缺陷。这表明沙门氏菌属。可能表达不止一种侵袭途径。Southern分析和染色体定位表明，多达9个染色体座位可能与入侵表型有关。越来越清楚的是，沙门氏菌的侵袭表型。与肠杆菌科的其他一些侵袭性成员相比，它是多因素和更复杂的。

There are an estimated 2 million cases of salmonellosis in the United States every year. Unlike the incidence of many infectious diseases, the incidence of salmonellosis in the United States and other developed countries has been rising steadily over the past 30 years, and the disease now accounts for 10 to 15% of all cases of acute gastroenteritis in the United States. The infecting organism is ingested and must traverse the intestinal epithelium to reach its preferred site for multiplication, the reticuloendothelial system. Despite several recent studies, the genetic basis of the invasion process is poorly understood. An emerging theme from these studies is that wild-type Salmonella organisms probably have several chromosomal loci that are required for the most efficient level of invasion. In this study, we have identified and characterized 13 TnphoA insertion mutants of Salmonella enteritidis CDC5 that exhibit altered invasion phenotypes. The mutants were identified by screening a bank of TnphoA insertions in S. enteritidis CDC5str for their invasion phenotype in three tissue culture cell lines (HEp-2, CHO, and MDCK). These 13 mutants were separated into six classes based on their invasive phenotypes in the tissue culture cell lines. Several mutants were defective for entry of some cell lines but not for others, while two mutants (SM6 and SM7) were defective for entry into all three tissue culture cell lines. This suggests that Salmonella spp. may express more than one invasion pathway. Southern analysis and chromosomal mapping indicated that as many as nine chromosomal loci may contribute to the invasion phenotype. It is becoming clear that the invasive phenotype of Salmonella spp. is multifactorial and more complex than that of some other invasive members of the family Enterobacteriaceae.