Mesoscopic Mapping of Ictal Neurovascular Coupling in Awake Behaving Mice Using Optical Spectroscopy and Genetically Encoded Calcium Indicators.
Mesoscopic Mapping of Ictal Neurovascular Coupling in Awake Behaving Mice Using Optical Spectroscopy and Genetically Encoded Calcium Indicators.
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使用光谱和遗传编码的钙指标在清醒的行为小鼠中对诊断神经血管耦合的介观映射。
DOI：
10.3389/fnins.2021.704834
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发表时间：
2021
影响因子：
4.3
通讯作者：
Schwartz TH
中科院分区：
文献类型：
--
作者：
Yang F;Li J;Song Y;Zhao M;Niemeyer JE;Luo P;Li D;Lin W;Ma H;Schwartz TH
以高空间分辨率明确识别癫痫病灶是一个挑战，特别是当没有解剖异常可以检测到。基于神经血管耦合（NVC）的脑标测技术经常应用于临床，尽管对发作NVC机制的理解很差，主要来自于对发作核心空间采样有限的麻醉动物的记录。在这项研究中，我们使用GCamp 6f和固有光学信号（IOS）的同时宽场介观成像来记录清醒、行为正常的小鼠在急性发作事件期间的神经元和血液动力学变化。比较异氟烷麻醉小鼠中的类似信号，以突出清醒状态的独特特征。在清醒的动物中，癫痫发作在发作时更集中，但更有可能传播到对侧半球。来自脑血容量（CBV）增加的HbT信号在清醒的小鼠中更强烈。结果，血红蛋白氧合的“癫痫性下降”作为标测信号变得不一致和不可靠。我们的数据表明，CBV为基础的成像技术应该是更准确的比血氧水平依赖（BOLD）为基础的成像技术癫痫发作映射清醒的行为动物。
Unambiguously identifying an epileptic focus with high spatial resolution is a challenge, especially when no anatomic abnormality can be detected. Neurovascular coupling (NVC)-based brain mapping techniques are often applied in the clinic despite a poor understanding of ictal NVC mechanisms, derived primarily from recordings in anesthetized animals with limited spatial sampling of the ictal core. In this study, we used simultaneous wide-field mesoscopic imaging of GCamp6f and intrinsic optical signals (IOS) to record the neuronal and hemodynamic changes during acute ictal events in awake, behaving mice. Similar signals in isoflurane-anesthetized mice were compared to highlight the unique characteristics of the awake condition. In awake animals, seizures were more focal at the onset but more likely to propagate to the contralateral hemisphere. The HbT signal, derived from an increase in cerebral blood volume (CBV), was more intense in awake mice. As a result, the “epileptic dip” in hemoglobin oxygenation became inconsistent and unreliable as a mapping signal. Our data indicate that CBV-based imaging techniques should be more accurate than blood oxygen level dependent (BOLD)-based imaging techniques for seizure mapping in awake behaving animals.
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影响因子：
4.6
作者：
Heo C;Park H;Kim YT;Baeg E;Kim YH;Kim SG;Suh M
通讯作者：
Suh M
影响因子：
3
作者：
ChenBee, CH;Kwon, MC;Frostig, RD
通讯作者：
Frostig, RD
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.7
作者：
Austin, VC;Blamire, AM;Sibson, NR
通讯作者：
Sibson, NR
影响因子：
8.8
作者：
FLYNN, NM;BULJUBASIC, N;KAMPINE, JP
通讯作者：
KAMPINE, JP