Complete genome sequencing of Lactobacillus plantarum CAUH2 reveals a novel plasmid pCAUH203 associated with oxidative stress tolerance
Complete genome sequencing of Lactobacillus plantarum CAUH2 reveals a novel plasmid pCAUH203 associated with oxidative stress tolerance
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植物乳杆菌 CAUH2 的完整基因组测序揭示了与氧化应激耐受性相关的新型质粒 pCAUH203
DOI：
10.1007/s13205-019-1653-4
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发表时间：
2019-03
期刊：
影响因子：
2.8
通讯作者：
Hao Yanling
中科院分区：
文献类型：
--
作者：
Zhai Zhengyuan;Yang Yang;Wang Jiaojiao;Wang Guohong;Ren Fazheng;Hao Yanling
植物乳杆菌对不同的生境具有显著的适应性，在食品工业中得到了广泛的应用。在本研究中，L。Plantarum CAUH2与其它L.植物基因组序列。CAUH2 Tol.基因组序列的比较PlantarumST-III与CAUH2的同源性高达99%，覆盖率达98%，但CAUH2和ST-III的质粒谱不同。值得注意的是，pCAUH203 INL.PlantarumCAUH2含有7个参与氧化应激反应的基因，如编码硫氧还蛋白-二硫键还原酶、硫氧还蛋白和DNA保护蛋白的基因。由于pCAUH203的存在，CAUH2的硫氧还蛋白还原酶活性是ST-III的2.1倍。当暴露于5 mM H_2O_2时，CAUH_2的这种活性进一步提高到9.87±1.60 mU/mg蛋白，是ST-III的2.7倍，表明pCAUH203编码的硫氧还蛋白抗氧化系统可能参与了H_2O_2的抗性。在10 mM H_2O_2胁迫下的存活实验进一步证实了这一假说。CAUH2细胞的存活率是ST-III细胞的12倍。因此，全基因组测序的L。PlantarumCAUH2为其抗氧化性的分子机制提供了新的见解。
Lactobacillus plantarumis remarkably adaptable to diverse habitats and is widely used in food industry. In this study, the genome sequence ofL. plantarumCAUH2 was analyzed and compared with otherL. plantarumgenome sequences. A comparison of the genome sequence of CAUH2 toL. plantarumST-III reveals that the similarity of these two genomes reached up to 99% identity with 98% coverage, but the plasmid profiles of CAUH2 and ST-III are different. Notably, plasmid pCAUH203 inL. plantarumCAUH2 harbors seven genes involved in oxidative stress response, such as genes encoding thioredoxin-disulfide reductase, thioredoxin and DNA protection protein. Due to plasmid pCAUH203, the thioredoxin reductase activity of CAUH2 was 2.1-fold higher than that of ST-III. When exposed to 5 mM H2O2, this activity was further increased to 9.87 ± 1.60 mU per mg protein in CAUH2, which was 2.7-fold higher than that of ST-III, indicating that thioredoxin antioxidant system encoded by pCAUH203 might contribute to the H2O2resistance. This hypothesis was further confirmed by survival assay under 10 mM H2O2stress. The survival rate of CAUH2 was 12-fold higher than that of ST-III. Therefore, the complete genome sequencing ofL. plantarumCAUH2 provides new insights into the molecular mechanism of its oxidative stress resistance.
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