Impact of natural genetic variation on the transcriptome of autotetraploid Arabidopsis thaliana
Impact of natural genetic variation on the transcriptome of autotetraploid Arabidopsis thaliana
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自然遗传变异对同源四倍体拟南芥转录组的影响
DOI：
10.1073/pnas.1000852107
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Zheng Yu
中科院分区：
文献类型：
--
作者：
Zheng Yu
多倍体，即生物体中存在两套以上完整的染色体，在进化过程中显著地塑造了被子植物的基因组。通常认为多倍体有两种形式：异源多倍体，源于种间杂交；自同源多倍体，源于种内基因组复制事件。除了影响基因组组织外，多倍体还产生其他遗传效应。合成的异源多倍体植物表现出相当大的转录组改变，其中部分可能是由于先前分化的调控等级的重新统一引起的。相比之下，自多倍体具有相对统一的基因组，表明基因表达的改变较低。为了评估种内基因组复制对转录组的影响，我们利用不同的生态型获得了一系列独特的拟南芥同源四倍体。拟南芥同源四倍体表现出转录组的改变，这种改变强烈依赖于它们亲本基因组的组成，包括先前从异源多倍体拟南芥中描述的新基因和基因群的表达改变。基因表达的改变是稳定的、非随机的、发育特异性的，并与DNA甲基化的变化有关。我们认为拟南芥具有一种固有的和可遗传的能力，可以感知和响应染色体数量的增加和平衡。自然变异对同源四倍体基因表达改变的影响强调了其在植物进化和育种中的潜在重要性。
Polyploidy, the presence of more than two complete sets of chromosomes in an organism, has significantly shaped the genomes of angiosperms during evolution. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies genome duplication events. Besides affecting genome organization, polyploidy generates other genetic effects. Synthetic allopolyploid plants exhibit considerable transcriptome alterations, part of which are likely caused by the reunion of previously diverged regulatory hierarchies. In contrast, autopolyploids have relatively uniform genomes, suggesting lower alteration of gene expression. To evaluate the impact of intraspecies genome duplication on the transcriptome, we generated a series of uniqueArabidopsis thalianaautotetraploids by using different ecotypes.A. thalianaautotetraploids show transcriptome alterations that strongly depend on their parental genome composition and include changed expression of both new genes and gene groups previously described from allopolyploidArabidopsis. Alterations in gene expression are stable, nonstochastic, developmentally specific, and associated with changes in DNA methylation. We propose thatArabidopsispossesses an inherent and heritable ability to sense and respond to elevated, yet balanced chromosome numbers. The impact of natural variation on alteration of autotetraploid gene expression stresses its potential importance in the evolution and breeding of plants.
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副突变：表观遗传指令代代相传。
影响因子：
3.3
作者：
Vicki L Chandler;Mary Alleman
通讯作者：
Mary Alleman
影响因子：
64.8
作者：
Zheng, Xianwu;Pontes, Olga;Zhu, Jianhua;Miki, Daisuke;Zhang, Fei;Li, Wen-Xue;Iida, Kei;Kapoor, Avnish;Pikaard, Craig S.;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
染色体植物学和栽培植物的起源
DOI：
--
发表时间：
1973
期刊：
影响因子：
--
作者：
C. D. Darlington
通讯作者：
C. D. Darlington
影响因子：
64.5
作者：
Zhang, Xiaoyu;Yazaki, Junshi;Ecker, Joseph R.
通讯作者：
Ecker, Joseph R.
影响因子：
56.9
作者：
Galitski, T;Saldanha, AJ;Fink, GR
通讯作者：
Fink, GR