Fetal cord blood and tissue immune responses to chronic placental inflammation and chorioamnionitis.
Fetal cord blood and tissue immune responses to chronic placental inflammation and chorioamnionitis.
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DOI：
10.1186/s13223-018-0297-y
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kumar R
中科院分区：
文献类型：
--
作者：
Singh AM;Sherenian MG;Kim KY;Erickson KA;Yang A;Mestan K;Ernst LM;Kumar R
绒毛膜炎是未来哮喘发展的危险因素。绒毛膜炎的动物模型显示与促炎细胞因子升高相关的TH 17与Treg比率增加。尚不清楚绒毛膜炎与新生儿免疫细胞系统性和组织内的相关性。我们招募了两个队列来评估绒毛膜炎中的TH 17和调节性T细胞（Treg）表型标志物。从一组19名活产婴儿中，我们收集了脐带血和胎盘样本，以评估急性和慢性组织学炎症和细胞表型特征的体征。我们分析了第二组有和没有绒毛膜炎的死产婴儿，对脾脏、胸腺和肺的细胞浸润表型进行分类和计数。我们使用线性回归分析确定视黄酸相关孤儿受体γ t阳性（RORγt+）和Treg细胞频率与活体队列受试者中观察到的不同类型炎症的相关性。使用线性混合模型，我们评估了绒毛膜炎与T细胞和B细胞之间的任何关联，细胞标志物的表达水平采用对数标度。然后，我们进行Wilcoxon秩和检验，以评估细胞计数和绒毛膜炎之间的关联。在患有慢性胎盘炎症的活产受试者中，与既无急性炎症也无慢性炎症的受试者相比，我们观察到Foxp 3+细胞中RORγt+细胞的比例增加，无论是否存在急性炎症。我们还发现，与没有急性或慢性炎症的受试者相比，患有急性高阶段胎儿和母体炎症的受试者中Foxp 3+细胞内RORγt+细胞的比例增加。在患有绒毛膜炎的死胎受试者中，我们观察到与没有绒毛膜炎的受试者相比，脾脏Foxp 3+细胞减少，肺CD 3+细胞增加。子宫内暴露于绒毛膜炎可能会影响新生儿的免疫激活，全身RORγt+细胞频率增加，以及肺中淋巴细胞浸润。我们的研究结果表明，在绒毛膜炎期间RORγt+细胞增加，因此可能支持绒毛膜炎与哮喘之间的已知关联。本文的在线版本（10.1186/s13223-018-0297-y）包含补充材料，可供授权用户使用。
Chorioamnionitis is a risk factor for future asthma development. Animal models of chorioamnionitis demonstrate increased TH17-to-Treg ratios associated with proinflammatory cytokine elevations. The association of chorioamnionitis on human neonatal immune cells systemically and within tissues is not known. We enrolled two cohorts to evaluate TH17 and regulatory T cell (Treg) phenotypic markers in chorioamnionitis. From a cohort of 19 live birth infants, we collected cord blood and placenta samples to evaluate for signs of acute and chronic histologic inflammation and cell phenotype characterization. We analyzed a second cohort of stillborn infants with and without chorioamnionitis to classify and enumerate cell infiltrate phenotypes in the spleen, thymus, and lung. We used linear regression analysis determine the association of retinoic acid-related orphan receptor gamma t positive (RORγt+) and Treg cell frequency with different types of inflammation seen in the live cohort subjects. Using linear mixed models, we evaluated for any associations between chorioamnionitis and T- and B-cell with a logarithmic scale for level of expression of cellular markers. We then performed Wilcoxon rank sum tests to assess the associations between cell count and chorioamnionitis. In the live birth subjects with chronic placental inflammation we observed an increased proportion of RORγt+ cells in Foxp3+ cells, regardless of the presence of acute inflammation, compared to subjects with neither acute nor chronic inflammation. We also found an increased proportion of RORγt+ cells within Foxp3+ cells in subjects with acute high stage fetal and maternal inflammation compared to those without acute or chronic inflammation. In the stillborn subjects with chorioamnionitis, we observed a decrease in splenic Foxp3+ cells and an increase in lung CD3+ cells compared with subjects that did not have chorioamnionitis. Exposure to chorioamnionitis in utero may affect immune activation in neonates with an increased frequency of RORγt+ cells systemically as well as lymphocytic infiltrate in the lung. Our findings suggest an increase in RORγt+ cells during chorioamnionitis and thus may support the known associations between chorioamnionitis with asthma. The online version of this article (10.1186/s13223-018-0297-y) contains supplementary material, which is available to authorized users.
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影响因子：
64.8
作者：
Bettelli, E;Carrier, YJ;Kuchroo, VK
通讯作者：
Kuchroo, VK
DOI：
10.4049/jimmunol.1300270
发表时间：
2013-08-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kallapur SG;Presicce P;Senthamaraikannan P;Alvarez M;Tarantal AF;Miller LM;Jobe AH;Chougnet CA
通讯作者：
Chougnet CA
影响因子：
3.7
作者：
Jones MC;Dye SR;Fernandes JA;Frölicher TL;Pinnegar JK;Warren R;Cheung WW
通讯作者：
Cheung WW
影响因子：
2.1
作者：
Tita AT;Andrews WW
通讯作者：
Andrews WW
影响因子：
64.8
作者：
Sakaguchi, N;Takahashi, T;Sakaguchi, S
通讯作者：
Sakaguchi, S