Genome-wide mutational diversity in an evolving population of Escherichia coli.
Genome-wide mutational diversity in an evolving population of Escherichia coli.
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DOI：
10.1101/sqb.2009.74.018
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Lenski RE
中科院分区：
文献类型：
--
作者：
Barrick JE;Lenski RE
种群中的遗传变异水平是进化力量之间动态紧张的结果。突变创造了变异，某些依赖于频率的相互作用可能保留了多样性，自然选择清除了变异。新的测序技术提供了前所未有的机会，在全基因组范围内发现和表征进化中的微生物种群的多样性。通过对混合种群样本进行测序，我们已经确定了在大肠杆菌种群历史上的不同时间点存在的单核苷酸多态，该种群从一个创始克隆进化了近20年。有了50倍的基因组覆盖，我们能够捕捉到有益的突变，因为它们席卷到固定，发现竞争的有益等位基因，被克隆干扰消除，并检测到其他可能适应新的生态位的次要变异。此外，在实验后期，在突变体表型进化后，遗传多样性有了显著的增加。在微生物进化实验中，更精细地分辨遗传变异的结构以及它是如何随时间变化的细节，将使种群遗传学理论的新应用和定量测试成为可能。
The level of genetic variation in a population is the result of a dynamic tension between evolutionary forces. Mutations create variation, certain frequency-dependent interactions may preserve diversity, and natural selection purges variation. New sequencing technologies offer unprecedented opportunities to discover and characterize the diversity present in evolving microbial populations on a whole-genome scale. By sequencing mixed-population samples, we have identified single-nucleotide polymorphisms present at various points in the history of an Escherichia coli population that has evolved for almost 20 years from a founding clone. With 50-fold genome coverage we were able to catch beneficial mutations as they swept to fixation, discover contending beneficial alleles that were eliminated by clonal interference, and detect other minor variants possibly adapted to a new ecological niche. Additionally, there was a dramatic increase in genetic diversity late in the experiment after a mutator phenotype evolved. Still finer resolution details of the structure of genetic variation and how it changes over time in microbial evolution experiments will enable new applications and quantitative tests of population genetic theory.
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影响因子：
2.9
作者：
LENSKI, RE;ROSE, MR;TADLER, SC
通讯作者：
TADLER, SC
DOI：
10.1073/pnas.91.15.6808
发表时间：
1994-07-19
影响因子：
11.1
作者：
LENSKI, RE;TRAVISANO, M
通讯作者：
TRAVISANO, M
影响因子：
56.9
作者：
de Visser, JAGM;Zeyl, CW;Lenski, RE
通讯作者：
Lenski, RE
影响因子：
2.9
作者：
Muller, HJ
通讯作者：
Muller, HJ
DOI：
10.1073/pnas.0801523105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Campbell, Peter J.;Pleasance, Erin D.;Stratton, Michael R.
通讯作者：
Stratton, Michael R.