Sendai virus gene sequences identified by oligonucleotide mapping.
Sendai virus gene sequences identified by oligonucleotide mapping.
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通过寡核苷酸作图鉴定仙台病毒基因序列。
DOI：
10.1016/0042-6822(82)90314-2
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发表时间：
1982
期刊：
影响因子：
3.7
通讯作者：
Kingsbury,DW
中科院分区：
文献类型：
--
作者：
Amesse,LS;Kingsbury,DW
我们已经使用T1-寡核苷酸图谱来探索仙台病毒基因组的核苷酸序列组织。通过琼脂糖凝胶电泳分离通过核糖核酸酶消化RNA杂交体获得的放射性病毒基因和未标记的病毒信使RNA种类的双链体。根据相对于病毒基因产物分子量的表观分子量推断双链体的身份。将五个双链体分配给定位在病毒基因组3′侧的五个基因。然而，寡核苷酸图谱显示，基因和双链体之间并不是简单的关系：（a）3′端基因NP存在于两个双链体中，其中较大的一个含有额外的寡核苷酸，可能代表病毒前导RNA的模板;（B）该组中最大的条带产生的寡核苷酸是任何其它双链体的两倍多，并且似乎代表一对双链体含有P和F0基因。分别鉴定了L基因的特征性寡核苷酸;它们相对于该基因分子量的丰度表明，与3′侧相比，病毒基因组的5′侧鸟嘌呤贫乏。还鉴定了病毒基因组全长互补序列（50 S正链）和正义病毒信使RNA的寡核苷酸。这些数据提供了进一步的结构分析的基因组的模型副粘病毒及其缺陷干扰（DI）缺失突变体的基础。
We have used Tl-oligonucleotide mapping to explore the nucleotide sequence organization of the Sendai virus genome. Duplexes of radioactive virus genes and unlabeled virus messenger RNA species, obtained by ribonuclease digestion of RNA hybrids, were separated by agarose gel electrophoresis. Identities of duplexes were deduced from their apparent molecular weights, relative to the molecular weights of the virus gene products. Five duplexes were assigned to the five genes that map at the 3′ side of the virus genome. However, oligonucleotide mapping revealed that there was not a simple relationship between genes and duplexes: (a) the 3′-terminal gene, NP, was present in two duplexes, the larger of which contained an additional oligonucleotide that may represent the template for the viral leader RNA; (b) the largest band in the group yielded more than twice as many oligonucleotides as any of the other duplexes and appears to represent a pair of duplexes containing the P and F0genes. The characteristic oligonucleotides of the L gene were identified separately; their abundance relative to the molecular weight of this gene suggested that the 5′ side of the viral genome is guanine-poor, compared to the 3′ side. Oligonucleotides of the full-length complement of the viral genome (50 S plus-strand) and of positive-sense virus messenger RNAs were also identified. These data provide a foundation for further structural analysis of the genome of this model paramyxovirus and its defective-interfering (DI) deletion mutants.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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影响因子：
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作者：
A. Portner;D. Kingsbury
通讯作者：
D. Kingsbury
DOI：
10.1073/pnas.73.5.1504
发表时间：
1976-01-01
影响因子：
11.1
作者：
ABRAHAM, G;BANERJEE, AK
通讯作者：
BANERJEE, AK