Depletion of Mdig Changes Proteomic Profiling in Triple Negative Breast Cancer Cells.
Depletion of Mdig Changes Proteomic Profiling in Triple Negative Breast Cancer Cells.
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DOI：
10.3390/biomedicines10082021
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发表时间：
2022-08-19
期刊：
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
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作者：
三阴性乳腺癌具有高度侵略性，总体预后不良和治疗选择有限。我们以前曾研究过MDIG的作用，MDIG是某些环境危险因素引起的致癌基因对乳腺癌发病机理的作用。然而，尚未确定对三阴性乳腺癌中MDIG影响的蛋白质组学特征的全面分析。使用无标签的自下而上的定量蛋白质组学，我们比较了WildType对照和MDIG敲除MDA-MB-231细胞，并鉴定了随着MDIG缺失显着改变的蛋白质和途径。在KO细胞中鉴定了总共904个差异表达（P <0.005）蛋白。与乳腺癌致病性相关的大约30条途径和网络被上调或下调，例如EIF2信号，未折叠的蛋白质反应和异亮氨酸降解I. Ingenition I. Ingenity途径分析确定，差异表达的蛋白质在细胞生长，运动性，运动性和恶性中具有相关的生物学作用。这些数据提供了与MDIG基因完全破坏有关的乳腺癌蛋白质表达模式的首次见解，并提供了有关MDIG调节的关键蛋白质，生物过程和途径的大量信息，这些信息促进了乳腺癌肿瘤性和侵入性。
Triple-negative breast cancers are highly aggressive with an overall poor prognosis and limited therapeutic options. We had previously investigated the role of mdig, an oncogenic gene induced by some environmental risk factors, on the pathogenesis of breast cancer. However, a comprehensive analysis of the proteomic profile affected by mdig in triple-negative breast cancer has not been determined yet. Using label-free bottom-up quantitative proteomics, we compared wildtype control and mdig knockout MDA-MB-231 cells and identified the proteins and pathways that are significantly altered with mdig deletion. A total of 904 differentially expressed (p < 0.005) proteins were identified in the KO cells. Approximately 30 pathways and networks linked to the pathogenicity of breast cancer were either up- or downregulated, such as EIF2 signaling, the unfolded protein response, and isoleucine degradation I. Ingenuity Pathway Analysis established that the differentially expressed proteins have relevant biological actions in cell growth, motility, and malignancy. These data provide the first insight into protein expression patterns in breast cancer associated with a complete disruption of the mdig gene and yielded substantial information on the key proteins, biological processes, and pathways modulated by mdig that contribute to breast cancer tumorigenicity and invasiveness.
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DOI：
10.1158/1078-0432.ccr-08-1402
发表时间：
2009-02-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Bedolla RG;Wang Y;Asuncion A;Chamie K;Siddiqui S;Mudryj MM;Prihoda TJ;Siddiqui J;Chinnaiyan AM;Mehra R;de Vere White RW;Ghosh PM
通讯作者：
Ghosh PM
影响因子：
3.7
作者：
Belin S;Beghin A;Solano-Gonzàlez E;Bezin L;Brunet-Manquat S;Textoris J;Prats AC;Mertani HC;Dumontet C;Diaz JJ
通讯作者：
Diaz JJ
影响因子：
3.4
作者：
Coughlin, MF;Puig-de-Morales, M;Fredberg, JJ
通讯作者：
Fredberg, JJ
影响因子：
8.8
作者：
Ferrandina, G;Scambia, G;Messori, A
通讯作者：
Messori, A
影响因子：
6.2
作者：
Baquero, Maria T.;Hanna, Jason A.;Neumeister, Veronique;Cheng, Huan;Molinaro, Annette M.;Harris, Lyndsay N.;Rimm, David L.
通讯作者：
Rimm, David L.