Carbohydrate cluster microarrays fabricated on three-dimensional dendrimeric platforms for functional glycomics exploration.
Carbohydrate cluster microarrays fabricated on three-dimensional dendrimeric platforms for functional glycomics exploration.
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DOI：
10.1021/pr900452s
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发表时间：
2009-11
影响因子：
4.4
通讯作者：
Wang, Denong
中科院分区：
文献类型：
--
作者：
Zhou, Xichun;Turchi, Craig;Wang, Denong
关键词：
We reported here a novel, ready-to-use bioarray platform and methodology for construction of sensitive carbohydrate cluster microarrays. This technology utilizes a 3-dimensional (3-D) poly(amidoamine) starburst dendrimer monolayer assembled on glass surface, which is functionalized with terminal aminooxy and hydrazide groups for site-specific coupling of carbohydrates. A wide range of saccharides, including monosaccharides, oligosaccharides and polysaccharides of diverse structures, are applicable for the 3-D bioarray platform without prior chemical derivatization. The process of carbohydrate coupling is effectively accelerated by microwave radiation energy. The carbohydrate concentration required for microarray fabrication is substantially reduced using this technology. Importantly, this bioarray platform presents sugar chains in defined orientation and cluster configurations. It is, thus, uniquely useful for exploration of the structural and conformational diversities of glyco-epitope and their functional properties.
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影响因子：
64.5
作者：
Geijtenbeek, TBH;Torensma, R;Figdor, CG
通讯作者：
Figdor, CG
影响因子：
46.9
作者：
Fukui, S;Feizi, T;Chai, WG
通讯作者：
Chai, WG
影响因子：
3.9
作者：
Carroll, GT;Wang, DN;Koberstein, JT
通讯作者：
Koberstein, JT
DOI：
10.1164/ajrccm/154.4_pt_2.s133
发表时间：
1996-10-01
影响因子：
24.7
作者：
Feizi, T;Loveless, RW
通讯作者：
Loveless, RW
影响因子：
3.6
作者：
Carrasco, MR;Brown, RT;Silva, O
通讯作者：
Silva, O