Identification and characterization of the Spodoptera Su(var) 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection.
Identification and characterization of the Spodoptera Su(var) 3-9 histone H3K9 trimethyltransferase and its effect in AcMNPV infection.
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斜纹夜蛾 Su(var) 3-9 组蛋白 H3K9 三甲基转移酶的鉴定和表征及其在 AcMNPV 感染中的作用
DOI：
10.1371/journal.pone.0069442
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhong J
中科院分区：
文献类型：
--
作者：
Li B;Li S;Yin J;Zhong J
克隆并鉴定了组蛋白H3-赖氨酸9(H3K9)三甲基转移酶基因SU(Var)3-9。果子树)，S。甜菜和S。礼仪。序列分析表明，SU(Var)3-9在进化上高度保守。Sf9细胞中SU(Var)3-9蛋白定位于细胞核，与组蛋白H3、异染色质蛋白1a(HP1a)和HP1b相互作用。体外实验发现，在27℃和37℃下，酶活性均呈剂量依赖性，27℃时酶活性较高。加入特异性抑制剂毛壳素可降低组蛋白甲基化水平和宿主染色质松弛。相反，SU(Var)3-9的过表达导致组蛋白甲基化水平增加和细胞基因组紧凑。在AcMNV感染的Sf9细胞中，SU(Var)3-9的转录在感染后期升高，但大多数细胞基因的mRNA水平下降。毛霉素预处理Sf9细胞可加快病毒DNA复制，并提高多种病毒基因的转录水平，而在Sf9细胞中，经SU(Var)3-9表达载体预转化后，病毒DNA复制略有减慢。这些发现表明，SU(Var)3-9可能参与了AcMNPV感染过程中病毒基因的表达和基因组复制的抑制。这为理解病毒与宿主的相互作用机制提供了新的视角。
Histone H3-lysine9 (H3K9) trimethyltransferase gene Su(var) 3-9 was cloned and identified in three Spodoptera insects, Spodoptera frugiperda ( S . frugiperda ), S . exigua and S . litura . Sequence analysis showed that Spodoptera Su(var) 3-9 is highly conserved evolutionarily. Su(var) 3-9 protein was found to be localized in the nucleus in Sf9 cells, and interact with histone H3, and the heterochromatin protein 1a (HP1a) and HP1b. A dose-dependent enzymatic activity was found at both 27 °C and 37 °C in vitro, with higher activity at 27 °C. Addition of specific inhibitor chaetocin resulted in decreased histone methylation level and host chromatin relaxation. In contrast, overexpression of Su(var) 3-9 caused increased histone methylation level and cellular genome compaction. In AcMNV-infected Sf9 cells, the transcription of Su(var) 3-9 increased at late time of infection, although the mRNA levels of most cellular genes decreased. Pre-treatment of Sf9 cells with chaetocin speeded up viral DNA replication, and increased the transcription level of a variety of virus genes, whereas in Sf9 cells pre-transformed with Su(var) 3-9 expression vector, viral DNA replication slow down slightly. These findings suggest that Su(var) 3-9 might participate in the viral genes expression an genome replication repression during AcMNPV infection. It provided a new insight for the understanding virus–host interaction mechanism.
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影响因子：
3.7
作者：
Boeke J;Regnard C;Cai W;Johansen J;Johansen KM;Becker PB;Imhof A
通讯作者：
Imhof A
影响因子：
3.8
作者：
Nobiron, I;O'Reilly, D;Olzewski, JA
通讯作者：
Olzewski, JA
影响因子：
2.9
作者：
Cosgrove, MS;Wolberger, C
通讯作者：
Wolberger, C
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
DOI：
10.1073/pnas.0908365106
发表时间：
2009-11-24
影响因子：
11.1
作者：
Belloni, Laura;Pollicino, Teresa;Levrero, Massimo
通讯作者：
Levrero, Massimo