Biological Uptake, Distribution, and Depuration of Radio-Labeled Graphene in Adult Zebrafish: Effects of Graphene Size and Natural Organic Matter.
Biological Uptake, Distribution, and Depuration of Radio-Labeled Graphene in Adult Zebrafish: Effects of Graphene Size and Natural Organic Matter.
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成年斑马鱼放射性标记石墨烯的生物吸收、分布和净化：石墨烯尺寸和天然有机物质的影响
DOI：
10.1021/acsnano.6b07982
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发表时间：
2017-03-28
期刊：
影响因子：
17.1
通讯作者：
Mao L
中科院分区：
文献类型：
--
作者：
Lu K;Dong S;Petersen EJ;Niu J;Chang X;Wang P;Lin S;Gao S;Mao L
石墨烯材料令人兴奋的商业应用潜力可能不可避免地导致它们越来越多地释放到环境中，从而可能造成生态风险。本研究利用碳-14标记的少层石墨烯（FLG）研究了石墨烯的大小对其在成年斑马鱼体内的吸收、净化和生物分布的影响。在暴露于250 μg/L的较大FLG（L-FLG）48小时后，生物体中石墨烯的量接近48 mg/kg鱼干质量，这是暴露于相同浓度的较小FLG（S-FLG）的那些人的身体负担的170倍以上。L-FLG和S-FLG的摄取量分别增加了2.5和16倍，当天然有机物（NOM）被添加到曝光悬浮液。虽然L-FLG主要积累在肠道中的成年斑马鱼，S-FLG被发现在肠道和肝脏暴露后与或没有NOM。引人注目的是，S-FLG能够穿过肠壁，进入肠上皮细胞和血液。NOM的存在增加了这些细胞中S-FLG的数量。暴露于L-FLG或S-FLG对肠道微生物群落结构也有显著不同的影响。
The exciting commercial application potential of graphene materials may inevitably lead to their increasing release into the environment where they may pose ecological risks. This study focused on using carbon-14 labeled few-layer graphene (FLG) to determine whether the size of graphene plays a role in its uptake, depuration and biodistribution in adult zebrafish. After 48 h exposure to larger FLG (L-FLG) at 250 μg/L, the amount of graphene in the organism was close to 48 mg/kg fish dry mass, which was more than 170-fold greater than the body burden of those exposed to the same concentration of smaller FLG (S-FLG). The amount of uptake for both L-FLG and S-FLG increased by a factor of 2.5 and 16, respectively, when natural organic matter (NOM) was added in the exposure suspension. While the L-FLG mainly accumulated in the gut of adult zebrafish, the S-FLG was found in both the gut and liver after exposure with or without NOM. Strikingly, the S-FLG was able to pass through the intestinal wall and enter the intestinal epithelial cells and blood. The presence of NOM increased the quantity of S-FLG in these cells. Exposure to L-FLG or S-FLG also had a significantly different impact on the intestinal microbial community structure.
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影响因子：
2.2
作者：
Almutary, A.;Sanderson, B. J. S.
通讯作者：
Sanderson, B. J. S.
影响因子：
4.5
作者：
Eide, Marta;Rusten, Marte;Goksoyr, Anders
通讯作者：
Goksoyr, Anders
影响因子：
17.1
作者：
Bertoli, Filippo;Garry, David;Dawson, Kenneth A.
通讯作者：
Dawson, Kenneth A.
影响因子：
9.4
作者：
HUNTER, PR;GASTON, MA
通讯作者：
GASTON, MA
影响因子：
41.2
作者：
Geim, A. K.;Novoselov, K. S.
通讯作者：
Novoselov, K. S.