iTRAQ-Based Phosphoproteomic Analysis of Toxoplasma gondii Tachyzoites Provides Insight Into the Role of Phosphorylation for its Invasion and Egress.
iTRAQ-Based Phosphoproteomic Analysis of Toxoplasma gondii Tachyzoites Provides Insight Into the Role of Phosphorylation for its Invasion and Egress.
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基于 iTRAQ 的弓形虫速殖子磷酸化蛋白质组学分析有助于深入了解磷酸化对其入侵和流出的作用
DOI：
10.3389/fcimb.2020.586466
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发表时间：
2020
影响因子：
5.7
通讯作者：
Liu ZZ
中科院分区：
文献类型：
--
作者：
He C;Xu MZ;Pan S;Wang H;Peng HJ;Liu ZZ
入侵和外出是裂解周期中对于弓形虫感染的传播至关重要的两个关键步骤，并且磷酸化被认为在这些过程中发挥着重要作用。然而，弓形虫在这两个阶段的磷酸蛋白质组尚未得到表征。在本研究中，我们基于iTRAQ定量分析对速殖子在“刚刚入侵”（JI）和“在离开之前”（PE）阶段的磷酸蛋白质组进行了分析，共检测到46个磷酸肽、42个磷酸化位点和38个磷蛋白。在PE与JI的比较中，检测到10个磷蛋白的磷酸化水平发生显着变化，其中4个在转录水平上被证明显着下调。对这些已鉴定的磷蛋白的生物信息分析表明，磷酸化介导的蛋白质功能调节用于调节弓形体病途径以及与速殖子的结合、定位和代谢相关的代谢和细胞过程，从而在寄生虫裂解周期中发挥重要作用。此外，生物信息学分析生成的蛋白质相互作用网络中，细胞骨架网络（CN）相关的内膜复合体（IMC1、IMC4、IMC6和IMC12）、血管内网络（IVN）相关的GRA（GRA2、GRA3、GRA7和GRA12）和寄生液泡膜（PVM）定位的ROP5在中心节点处富集，其中IMC4、GRA2、GRA3和GRA12的磷酸化水平被发现受到显着调节。该研究揭示了弓形虫出入的主要细胞过程和关键磷蛋白，将为弓形虫体外发育生物学提供新的见解，并有助于从寄生虫的角度理解病原体与宿主的相互作用。
The invasion and egress are two key steps in lytic cycle vital to the propagation of Toxoplasma gondii infection, and phosphorylation is believed to play important roles in these processes. However, the phosphoproteome of T. gondii at these two stages has not been characterized. In this study, we profiled the phosphoproteome of tachyzoites at the stages of “just invading” (JI) and “prior to egress” (PE) based on iTRAQ quantitative analysis, in which a total of 46 phosphopeptides, 42 phosphorylation sites, and 38 phosphoproteins were detected. In the comparison of PE vs. JI, 10 phosphoproteins were detected with their phosphorylation level significantly changed, and four of them were demonstrated to be significantly down-regulated at the transcriptional level. Bioinformatic analysis of these identified phosphoproteins suggested that phosphorylation-mediated modulation of protein function was employed to regulate the pathway of toxoplasmosis and metabolism and cellular processes correlated with tachyzoite’s binding, location, and metabolism, and thus play vital roles in the parasite lytic cycle. Moreover, cytoskeletal network (CN)-associated Inner Membrane Complex (IMC1, IMC4, IMC6 and IMC12), Intravascular Network (IVN)-related GRAs (GRA2, GRA3, GRA7 and GRA12), and Parasitophorous Vacuole Membrane (PVM)-localized ROP5 were shown to be enriched at the central nodes in the protein interaction network generated by bioinformatic analysis, in which the phosphorylation level of IMC4, GRA2, GRA3, and GRA12 were found to be significantly regulated. This study revealed the main cellular processes and key phosphoproteins crucial for the invasion and egress of T. gondii, which will provide new insights into the developmental biology of T. gondii in vitro and contribute to the understanding of pathogen-host interaction from the parasite perspective.
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弓形虫的裂解周期：15年后。
影响因子：
10.5
作者：
Blader IJ;Coleman BI;Chen CT;Gubbels MJ
通讯作者：
Gubbels MJ
影响因子：
14.9
作者：
Ma J;Chen T;Wu S;Yang C;Bai M;Shu K;Li K;Zhang G;Jin Z;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
3.8
作者：
Hotez PJ
通讯作者：
Hotez PJ
并不是一个简单的束缚：在入侵期间弓形虫Gondii AMA1与RON2的结合可保护AMA1免受菱形介导的裂解，并导致其胞质尾巴的去磷酸化。
影响因子：
6.4
作者：
Krishnamurthy S;Deng B;Del Rio R;Buchholz KR;Treeck M;Urban S;Boothroyd J;Lam YW;Ward GE
通讯作者：
Ward GE
DOI：
10.1007/978-1-4939-9857-9_23
发表时间：
2020-01-01
期刊：
TOXOPLASMA GONDII: METHODS AND PROTOCOLS
影响因子：
--
作者：
Broncel, Malgorzata;Treeck, Moritz
通讯作者：
Treeck, Moritz