Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues.
Tissue-specific melt electrowritten polymeric scaffolds for coordinated regeneration of soft and hard periodontal tissues.
复制标题
DOI：
10.1016/j.bioactmat.2022.04.013
复制
发表时间：
2023-01
影响因子：
18.9
通讯作者：
Bottino, Marco C.
中科院分区：
文献类型：
--
作者：
Daghrery, Arwa;Ferreira, Jessica A.;Xu, Jinping;Golafshan, Nasim;Kaigler, Darnell;Bhaduri, Sarit B.;Malda, Jos;Castilho, Miguel;Bottino, Marco C.
牙周炎是一种慢性炎症，通常会对牙齿支持组织造成严重损害。临床可用方法的成功结果有限，这凸显了对不仅能为细胞提供结构指导而且还能调节局部免疫反应的治疗方法的需求。在这里，具有组织特异性属性的三维熔融电写（即聚（ε-己内酯））支架被设计用于引导人源性牙周膜干细胞（hPDLSC）的分化并介导巨噬细胞极化。研究的组织特异性支架属性包括纤维形态（对齐与随机）和具有不同链间距（小 250 μm 和大 500 μm）的高度有序结构。巨噬细胞在对齐和高度有序的支架中表现出细长的形态，同时在随机定向的纤维支架上保持其圆形形状。骨膜素和 IL-10 的表达在对齐且高度有序的支架上更为明显。虽然链间距为 500 μm 的支架上的 hPDLSC 在 21 天内显示出较高的成骨标记物 (Runx2) 表达，但随机取向的纤维支架上的细胞显示出 M1 标记物的上调。在原位下颌开窗缺损模型中，研究结果表明，组织特异性支架（即用于牙周膜的对齐纤维和用于骨的高度有序的 500 μm 线间距氟化磷酸钙 [F/CaP] 涂层纤维）可以增强对天然牙周组织再生的模仿。通过熔体电写设计了聚合物组织特异性支架。组织特异性支架支持韧带生成和成骨分化。组织特异性支架介导对巨噬细胞（M2）的免疫调节作用。组织特异性支架引导体内软/硬牙周组织再生。
Periodontitis is a chronic inflammatory condition that often causes serious damage to tooth-supporting tissues. The limited successful outcomes of clinically available approaches underscore the need for therapeutics that cannot only provide structural guidance to cells but can also modulate the local immune response. Here, three-dimensional melt electrowritten (i.e., poly(ε-caprolactone)) scaffolds with tissue-specific attributes were engineered to guide differentiation of human-derived periodontal ligament stem cells (hPDLSCs) and mediate macrophage polarization. The investigated tissue-specific scaffold attributes comprised fiber morphology (aligned vs. random) and highly-ordered architectures with distinct strand spacings (small 250 μm and large 500 μm). Macrophages exhibited an elongated morphology in aligned and highly-ordered scaffolds, while maintaining their round-shape on randomly-oriented fibrous scaffolds. Expressions of periostin and IL-10 were more pronounced on the aligned and highly-ordered scaffolds. While hPDLSCs on the scaffolds with 500 μm strand spacing show higher expression of osteogenic marker (Runx2) over 21 days, cells on randomly-oriented fibrous scaffolds showed upregulation of M1 markers. In an orthotopic mandibular fenestration defect model, findings revealed that the tissue-specific scaffolds (i.e., aligned fibers for periodontal ligament and highly-ordered 500 μm strand spacing fluorinated calcium phosphate [F/CaP]-coated fibers for bone) could enhance the mimicking of regeneration of natural periodontal tissues. Polymeric tissue-specific scaffolds were engineered via melt electrowriting. Tissue-specific scaffolds supported ligamentogenic and osteogenic differentiation. Tissue-specific scaffolds mediated an immunomodulatory effect on macrophages (M2). Tissue-specific scaffolds guided in vivo soft/hard periodontal tissue regeneration.
登录
查看更多内容
影响因子：
9.5
作者：
Hu Z;Ma C;Rong X;Zou S;Liu X
通讯作者：
Liu X
影响因子：
11.3
作者：
Abbasi N;Lee RSB;Ivanovski S;Love RM;Hamlet S
通讯作者：
Hamlet S
影响因子：
11.3
作者：
Abbasi, Naghmeh;Ivanovski, Saso;Hamlet, Stephen
通讯作者：
Hamlet, Stephen
影响因子：
9.7
作者：
Dubey, Nileshkumar;Ferreira, Jessica A.;Bottino, Marco C.
通讯作者：
Bottino, Marco C.
影响因子：
9.7
作者：
Jiang, Wenlu;Li, Long;Zhou, Shaobing
通讯作者：
Zhou, Shaobing