Detection of SARS-CoV-2 in saliva and characterization of oral symptoms in COVID-19 patients.
Detection of SARS-CoV-2 in saliva and characterization of oral symptoms in COVID-19 patients.
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DOI：
10.1111/cpr.12923
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发表时间：
2020-12
影响因子：
8.5
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Chen L;Zhao J;Peng J;Li X;Deng X;Geng Z;Shen Z;Guo F;Zhang Q;Jin Y;Wang L;Wang S
为了更全面地了解SARS - CoV - 2对口腔健康的影响和可能的唾液传播，我们从公共数据库中进行了RNA - seq谱分析，并对COVID - 19患者的口腔相关症状进行了问卷调查。为了分析唾液腺中ACE2的表达，研究人员招募了来自四个公共数据集的大量RNA - seq图谱，其中包括31名COVID - 19患者。采集唾液和口咽拭子。采用实时聚合酶链反应（RT - PCR）检测唾液中SARS - CoV - 2核酸。此外，还对COVID - 19患者进行了口干和听力障碍等各种口腔症状的问卷调查。唾液腺中ACE2的表达可检测到。此外，在4例唾液SARS - CoV - 2核酸检测阳性的病例中，有3例（75%）危重，需要呼吸机支持。此外，我们观察到108名接受问卷调查的COVID - 19患者中有较高比例表现出两种主要的口腔相关症状，即口干（46.3%）和听力障碍（47.2%）。本研究证实了ACE2在唾液腺中的表达，并证明了SARS - CoV - 2感染唾液腺的可能性。唾液可能是危重病人诊断标本的新来源，因为它可以很容易地收集而不需要任何侵入性程序。此外，口干和四肢无力可被认为是COVID - 19感染的初始症状。本研究在通过数据库分析确认唾液腺中ACE2受体表达的基础上，假设并证实了唾液样本中可以检测到SARS‐COV‐2的RNA。同时，我们还在临床观察到SARS - COV - 2感染的初始口腔相关症状可能是听力障碍和口干。
In order to provide a more comprehensive understanding of the effects of SARS‐CoV‐2 on oral health and possible saliva transmission, we performed RNA‐seq profiles analysis from public databases and also a questionnaire survey on oral‐related symptoms of COVID‐19 patients. To analyse ACE2 expression in salivary glands, bulk RNA‐seq profiles from four public datasets including 31 COVID‐19 patients were recruited. Saliva and oropharyngeal swabs were collected. SARS‐CoV‐2 nucleic acids in saliva were detected by real‐time polymerase chain reaction (RT‐PCR). Additionally, a questionnaire survey on various oral symptoms such as dry mouth and amblygeustia was also carried out on COVID‐19 patients. ACE2 expression was present at detectable levels in the salivary glands. In addition, of four cases with positive detection of salivary SARS‐CoV‐2 nucleic acids, three (75%) were critically ill on ventilator support. Furthermore, we observed the two major oral‐related symptoms, dry mouth (46.3%) and amblygeustia (47.2%), were manifested by a relatively high proportion of 108 COVID‐19 patients who accepted the questionnaire survey. This study confirms the expression of ACE2 in the salivary glands and demonstrates the possibility of SARS‐CoV‐2 infection of salivary glands. Saliva may be a new source of diagnostic specimens for critically ill patients, since it can be easily collected without any invasive procedures. In addition, dry mouth and amblygeustia can be considered as initial symptoms of COVID‐19 infection. In this study, based on the confirmation of ACE2 receptor expression in salivary glands through database analysis, it was assumed and confirmed that RNA of SARS‐COV‐2 could be detected in saliva samples. At the same time, we also clinically observed that the initial oral‐related symptoms of SARS‐COV‐2 infection may be amblygeustia and dry mouth.
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