MicroRNA-mediated responses to long-term magnesium-deficiency in Citrus sinensis roots revealed by Illumina sequencing.
MicroRNA-mediated responses to long-term magnesium-deficiency in Citrus sinensis roots revealed by Illumina sequencing.
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Illumina 测序揭示了 MicroRNA 介导的柑橘根对长期缺镁的反应
DOI：
10.1186/s12864-017-3999-5
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发表时间：
2017-08-24
期刊：
影响因子：
4.4
通讯作者：
Chen LS
中科院分区：
文献类型：
--
作者：
Liang WW;Huang JH;Li CP;Yang LT;Ye X;Lin D;Chen LS
镁 (Mg) 缺乏症最常发生在强酸性沙质土壤中。柑橘主要生长在酸性和强酸性土壤上。缺镁会导致一些柑橘园的果实品质差和产量低。我们首次使用 Illumina 测序技术研究了‘雪柑’根部中缺镁反应的 miRNA，以获得一些可能与柑橘缺镁耐受性相关的 miRNA。我们从缺镁的根中获得了 101 (69) 个表达增加（减少）的 miRNA。我们的研究结果表明，柑橘根部对缺镁的适应与以下几个方面有关：（a）通过诱导miR158和miR2919抑制根部呼吸和相关基因表达； (b) 通过下调相关 miRNA（miR780、miR6190、miR1044、miR5261 和 miR1151）和适应低磷（miR6190）来增强抗氧化系统； (c)通过改变miR6190、miR6485、miR1044、miR5029和miR3437的表达来激活转运相关基因； (d)由于miR1044、miR5261、miR1151和miR5029的表达水平降低而提高蛋白质泛素化； (e)通过调节miR5261、miR6485和miR158表达维持根生长； (f)通过调节miR5176和miR6485（miR6028、miR6190、miR6485、miR5621、miR160和miR7708）表达来触发DNA修复（转录调控）。参与根部信号转导的缺镁响应 miRNA 在柑橘缺镁耐受性中也具有功能。我们获得了几种新的镁缺乏反应性 miRNA（即 miR5261、miR158、miR6190、miR6485、miR1151 和 miR1044），可能有助于镁缺乏耐受性。这些结果揭示了高等植物中 miRNA 介导的对营养缺乏的适应的一些新线索。
Magnesium (Mg)-deficiency occurs most frequently in strongly acidic, sandy soils. Citrus are grown mainly on acidic and strong acidic soils. Mg-deficiency causes poor fruit quality and low fruit yield in some Citrus orchards. For the first time, we investigated Mg-deficiency-responsive miRNAs in ‘Xuegan’ (Citrus sinensis) roots using Illumina sequencing in order to obtain some miRNAs presumably responsible for Citrus Mg-deficiency tolerance. We obtained 101 (69) miRNAs with increased (decreased) expression from Mg-starved roots. Our results suggested that the adaptation of Citrus roots to Mg-deficiency was related to the several aspects: (a) inhibiting root respiration and related gene expression via inducing miR158 and miR2919; (b) enhancing antioxidant system by down-regulating related miRNAs (miR780, miR6190, miR1044, miR5261 and miR1151) and the adaptation to low-phosphorus (miR6190); (c) activating transport-related genes by altering the expression of miR6190, miR6485, miR1044, miR5029 and miR3437; (d) elevating protein ubiquitination due to decreased expression levels of miR1044, miR5261, miR1151 and miR5029; (e) maintaining root growth by regulating miR5261, miR6485 and miR158 expression; and (f) triggering DNA repair (transcription regulation) by regulating miR5176 and miR6485 (miR6028, miR6190, miR6485, miR5621, miR160 and miR7708) expression. Mg-deficiency-responsive miRNAs involved in root signal transduction also had functions in Citrus Mg-deficiency tolerance. We obtained several novel Mg-deficiency-responsive miRNAs (i.e., miR5261, miR158, miR6190, miR6485, miR1151 and miR1044) possibly contributing to Mg-deficiency tolerance. These results revealed some novel clues on the miRNA-mediated adaptation to nutrient deficiencies in higher plants.
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