Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous Yeast Lipomyces starkeyi.
Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous Yeast Lipomyces starkeyi.
复制标题
柠檬酸盐介导的酰基辅酶A合成是促进产油酵母斯达氏油脂酵母生长和三酰甘油产生所必需的。
DOI：
10.3390/microorganisms9081693
复制
发表时间：
2021-08-09
期刊：
影响因子：
4.5
通讯作者：
Takaku H
中科院分区：
文献类型：
--
作者：
Sato R;Ara S;Yamazaki H;Ishiya K;Aburatani S;Takaku H
斯达氏油脂酵母是一种优良的三酰甘油（TAG）生产菌，可作为生物柴油生产的原料。为了了解TAG合成的调控，本研究中，我们试图分离具有降低的脂质生产力的突变体并分析TAG合成相关基因的表达。通过Percoll密度梯度离心法筛选，获得了一株产脂能力显著下降的突变株sr 22。与野生型菌株相比，sr 22突变体中柠檬酸介导的酰基辅酶A合成相关基因（ACL 1、ACL 2、ACC 1、FAS 1和FAS 2）的表达降低。同时提出了L.具有增加的脂质生产力的starkeyi突变体具有增加的基因表达，这些基因的表达与TAG合成之间存在相关性。为了阐明柠檬酸介导的酰基辅酶A合成途径对TAG合成的重要性，我们还构建了一株不含负责柠檬酸介导的酰基辅酶A合成第一反应的ATP-柠檬酸裂解酶的菌株，并研究了ATP-柠檬酸裂解酶对TAG合成的重要性。在葡萄糖培养基中，ATP-柠檬酸裂解酶是促进细胞生长和TAG合成所必需的。本研究为开发一种高效的TAG合成方法生产生物柴油提供了可能。
The oleaginous yeast Lipomyces starkeyi is an excellent producer of triacylglycerol (TAG) as a feedstock for biodiesel production. To understand the regulation of TAG synthesis, we attempted to isolate mutants with decreased lipid productivity and analyze the expression of TAG synthesis-related genes in this study. A mutant with greatly decreased lipid productivity, sr22, was obtained by an effective screening method using Percoll density gradient centrifugation. The expression of citrate-mediated acyl-CoA synthesis-related genes (ACL1, ACL2, ACC1, FAS1, and FAS2) was decreased in the sr22 mutant compared with that of the wild-type strain. Together with a notion that L. starkeyi mutants with increased lipid productivities had increased gene expression, there was a correlation between the expression of these genes and TAG synthesis. To clarify the importance of citrate-mediated acyl-CoA synthesis pathway on TAG synthesis, we also constructed a strain with no ATP-citrate lyase responsible for the first reaction of citrate-mediated acyl-CoA synthesis and investigated the importance of ATP-citrate lyase on TAG synthesis. The ATP-citrate lyase was required for the promotion of cell growth and TAG synthesis in a glucose medium. This study may provide opportunities for the development of an efficient TAG synthesis for biodiesel production.
登录
查看更多内容
影响因子：
2.8
作者：
Juanssilfero, Ario B.;Kahar, Prihardi;Kondo, Akihiko
通讯作者：
Kondo, Akihiko
影响因子：
8.4
作者：
Liu, Leqian;Markham, Kelly;Alper, Hal S.
通讯作者：
Alper, Hal S.
影响因子：
5
作者：
Matsuzawa, Tomohiko;Kamisaka, Yasushi;Yaoi, Katsuro
通讯作者：
Yaoi, Katsuro
影响因子：
6.4
作者：
Signori L;Ami D;Posteri R;Giuzzi A;Mereghetti P;Porro D;Branduardi P
通讯作者：
Branduardi P
影响因子：
2.7
作者：
Guo, Minrui;Cheng, Shaobo;Chen, Jiluan
通讯作者：
Chen, Jiluan