Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus
Characterization of two monoclonal antibodies, 38F10 and 44D11, against the major envelope fusion protein of Helicoverpa armigera nucleopolyhedrovirus
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抗棉铃虫核多角体病毒主要包膜融合蛋白的两种单克隆抗体 38F10 和 44D11 的表征
DOI：
10.1007/s12250-016-3831-4
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发表时间：
2016-12
影响因子：
5.5
通讯作者：
Zhang T
中科院分区：
文献类型：
--
作者：
Zou Z;Liu J;Wang Z;Deng F;Wang H;Hu Z;Wang M;Zhang T
The envelope fusion protein F of baculoviruses is a class I viral fusion protein which play a significant role during virus entry into insect cells. F is initially synthesized as a precursor (F0) and then cleaved into a disulfide-linked F1 and F2 subunits during the process of protein maturation and secretion. To facilitate further investigation into the structure and function of F protein during virus infection, monoclonal antibodies (mAbs) against the F2 subunit of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (HaF) were generated. Two kinds of mAbs were obtained according to their different recognition epitopes: one kind of mAbs, as represented by 38F10, recognizes amino acid (aa) 85 to 123 of F2 and the other kind, represented by 44D11, recognizes aa 148 to 173 of F2. Western blot and immunofluorescence assay confirmed that both of the mAbs recognized the F protein expressed in HearNPV infected cells, however, only 44D11 could neutralize HearNPV infection. The results further showed that 44D11 may not interact with a receptor binding epitope, rather it was demonstrated to inhibit syncytium formation in cells expressing the HaF protein.
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影响因子：
5.4
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Manli Wang;Jue Wang;F. Yin;Ying Tan;F. Dèng;Xinwen Chen;J. Jehle;J. Vlak;Zhìhóng Hú;Huálín Wáng
通讯作者：
Manli Wang;Jue Wang;F. Yin;Ying Tan;F. Dèng;Xinwen Chen;J. Jehle;J. Vlak;Zhìhóng Hú;Huálín Wáng
影响因子：
3.7
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L. Volkman;P. Goldsmith
通讯作者：
L. Volkman;P. Goldsmith
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Chen, XW;Zhang, WJ;Vlak, JM
通讯作者：
Vlak, JM
影响因子：
3.4
作者：
Bulach, DM;Kumar, CA;Tribe, DE
通讯作者：
Tribe, DE
影响因子：
5.4
作者：
MONSMA, SA;BLISSARD, GW
通讯作者：
BLISSARD, GW