Left ventricular geometry, tissue composition, and residual stress in High Fat Diet Dahl-Salt sensitive rats.
Left ventricular geometry, tissue composition, and residual stress in High Fat Diet Dahl-Salt sensitive rats.
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DOI：
10.1007/s11340-020-00664-8
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发表时间：
2021-01
影响因子：
2.4
通讯作者：
Roccabianca S
中科院分区：
文献类型：
--
作者：
Grobbel MR;Lee LC;Watts SW;Fink GD;Roccabianca S
高血压驱动心肌重塑，导致结构、成分和机械行为的变化，包括残余应力，这些变化以性别特定的方式与心脏病进展有关。新兴疗法也针对成分特异性病理特征。然而，所有先前的研究都描述了完整组织中的重塑，而不是分离的组织成分，并且没有将性别作为生物学变量。在本研究中，我们首次发现了胶原纤维网络和肌细胞对高脂饲料喂养的Dahl-Salt敏感大鼠心肌残余应激/应变的贡献。然后，我们量化了高血压对左心室（LV）重构的影响，以及存在性别特异性重构特征。我们对左室分离成分和完整组织进行了力学试验（开角试验、环试验）和组织学分析。根据测试的测量结果，我们进行了应力分析以评估残余应力分布。进行统计分析以确定成分分离、血压升高和动物性别对实验测量和建模结果输出的影响。高血压导致雄性和雌性大鼠的残余应力/应变减少，完整的组织，分离的胶原纤维和分离的肌细胞。在正常和高血压动物中，胶原蛋白仍然是心肌残余应激的最大贡献者。我们在高血压和正常血压的动物中都发现了性别差异。我们观察了高血压动物模型左室的成分和性别特异性重构特征。
Hypertension drives myocardial remodeling, leading to changes in structure, composition and mechanical behavior, including residual stress, which are linked to heart disease progression in a gender-specific manner. Emerging therapies are also targeting constituent-specific pathological features. All previous studies, however, have characterized remodeling in the intact tissue, rather than isolated tissue constituents, and did not include sex as a biological variable. In this study we first identified the contribution of collagen fiber network and myocytes to the myocardial residual stress/strain in Dahl-Salt sensitive rats fed with high fat diet. Then, we quantified the effect of hypertension on the remodeling of the left ventricle (LV), as well as the existence of sex-specific remodeling features. We performed mechanical tests (opening angle, ring-test) and histological analysis on isolated constituents and intact tissue of the LV. Based on the measurements from the tests, we performed a stress analysis to evaluate the residual stress distribution. Statistical analysis was performed to identify the effects of constituent isolation, elevated blood pressure, and sex of the animal on the output of both experimental measures and modeling results. Hypertension leads to reduced residual stress/strain intact tissue, isolated collagen fibers, and isolated myocytes in male and female rats. Collagen remains the largest contributor to myocardial residual stress in both normotensive and hypertensive animals. We identified sex-differences in both hypertensive and normotensive animals. We observed both constituent- and sex-specific remodeling features in the LV of an animal model of hypertension.
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影响因子：
3.5
作者：
Grobbel MR;Shavik SM;Darios E;Watts SW;Lee LC;Roccabianca S
通讯作者：
Roccabianca S
与性别相关的心力衰竭差异，保留的射血分数。
影响因子：
4.6
作者：
Duca F;Zotter-Tufaro C;Kammerlander AA;Aschauer S;Binder C;Mascherbauer J;Bonderman D
通讯作者：
Bonderman D
DOI：
10.1161/hypertensionaha.118.11485
发表时间：
2018-11
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Fernandes R;Garver H;Harkema JR;Galligan JJ;Fink GD;Xu H
通讯作者：
Xu H
影响因子：
3.5
作者：
Omens, J. H.;McCulloch, A. D.;Criscione, J. C.
通讯作者：
Criscione, J. C.
影响因子：
20.1
作者：
OMENS, JH;FUNG, YC
通讯作者：
FUNG, YC