Fine mapping of Pi57(t) conferring broad spectrum resistance against Magnaporthe oryzae in introgression line IL-E1454 derived from Oryza longistaminata.
Fine mapping of Pi57(t) conferring broad spectrum resistance against Magnaporthe oryzae in introgression line IL-E1454 derived from Oryza longistaminata.
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Pi57(t)的精细定位赋予来自长雄稻的基因渗入系IL-E1454对稻瘟病菌的广谱抗性
DOI：
10.1371/journal.pone.0186201
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Yang Q
中科院分区：
文献类型：
--
作者：
Dong L;Liu S;Xu P;Deng W;Li X;Tharreau D;Li J;Zhou J;Wang Q;Tao D;Yang Q
稻属野生种是改良亚洲栽培稻农艺性状的优良基因库。水稻侵染系IL-E1454的抗稻瘟病基因Pi57(t)已被定位到水稻12号染色体上。接种从6个国家收集的322株稻瘟病菌表明，Pi57(t)对稻瘟病菌具有广谱抗性。利用抗性供体IL-E1454与敏感亲本RD23和丽江新团黑谷杂交的29070和10375 F2两个定位群体对Pi57(t)位点进行了精细定位。根据从两个杂交中筛选的重组体的基因分型和表型结果，最终将Pi57(t)定位在12号染色体短臂上靠近着丝粒的51.7 kb区域，该区域两侧有两个分子标记（STS57-320和STS57-372）。根据Nipponbare的参考序列，在靶区预测了6个候选抗性基因。这些结果为抗病育种的标记辅助选择和基因克隆提供了理论依据。
Wild species of the genus Oryza are excellent gene pools for improvement of agronomic traits of Asian cultivated rice. The blast resistance gene Pi57(t) in the introgression line IL-E1454 derived from Oryza longistaminata was previously mapped on rice chromosome 12. Inoculation with 322 Magnaporthe oryzae isolates collected from 6 countries indicated that Pi57(t) conferred broad spectrum resistance against M. oryzae. Two mapping populations consisting of 29070 and 10375 F2 plants derived from the crosses of resistant donor IL-E1454 with susceptible parents RD23 and Lijiangxintuanheigu respectively, were used for fine mapping of Pi57(t) locus. Based on genotyping and phenotyping results of recombinants screened from the two crosses, Pi57(t) was finally mapped to a 51.7-kb region flanked by two molecular markers (STS57-320 and STS57-372) on the short arm and close to the centromere of chromosome 12. Six candidate resistance genes were predicted in the target region according to the reference sequence of Nipponbare. These results could facilitate both marker-assisted selection for disease-resistant breeding and gene cloning.
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