Serum metabonomic analysis of apoE(-/-) mice reveals progression axes for atherosclerosis based on NMR spectroscopy.
Serum metabonomic analysis of apoE(-/-) mice reveals progression axes for atherosclerosis based on NMR spectroscopy.
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对 apoE(-/-) 小鼠的血清代谢组学分析揭示了基于核磁共振波谱的动脉粥样硬化进展轴。
DOI：
10.1039/c4mb00334a
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Guo, Jiao
中科院分区：
文献类型：
--
作者：
Li, Xiaoqiang;Yuan, Fengying;Wang, Lijing;Guo, Jiao
动脉粥样硬化是一种多因素的进行性疾病，通常与高脂肪饮食有关。本研究的目的是使用（1）基于H NMR的代谢组学鉴定用于早期诊断和监测apoE（-/-）小鼠动脉粥样硬化形成进展的潜在生物标志物。将apoE（-/-）小鼠按高脂喂养时间分为4组（0、2、4、8 w），每组小鼠的病理特征不同。（1）基于血清NMR的代谢组学选择性地捕获与动脉粥样硬化程度相关的代谢型，显示从生理状态到病理生理状态的时间依赖性进展。高密度脂蛋白、胆碱、牛磺酸、甘氨酸和葡萄糖的变化可作为动脉粥样硬化早期的特异性生物标志物。随着动脉粥样硬化的进展，当动脉粥样硬化斑块较大时，出现缬氨酸、丙氨酸和蛋氨酸等氨基酸代谢紊乱。在apoE（-/-）小鼠动脉粥样硬化的进展中观察到涉及脂质代谢、能量和脂肪酸代谢的多种生化紊乱。本研究表明：（1）基于1H NMR的代谢组学可以提供动脉粥样硬化形成过程的生化信息，为发现动脉粥样硬化发生和发展的潜在生物标志物提供了一种无创的手段。
Atherosclerosis is a multifactorial and progressive disease commonly correlated with a high fat diet. The aim of this study was to identify potential biomarkers for the early diagnosis and monitoring of the progression of atherogenesis in apoE(-/-) mice using (1)H NMR-based metabonomics. The apoE(-/-) mice were split into four groups according to the duration of high fat feeding (0 w, 2 w, 4 w and 8 w), and each group possessed different pathological characteristics. Serum (1)H NMR-based metabonomics selectively captured the metabotypes that correlated with the degree of atherosclerosis, showing a time-dependent progression from the physiological to pathophysiological status. It was noted that changes in HDL, choline, taurine, glycine and glucose may be regarded as specific biomarkers of the early stage of atherosclerosis. With the progression of atherosclerosis, disorders in the metabolism of amino acids such as valine, alanine and methionine appeared when large atherosclerotic plaques existed. Multiple biochemical disorders involving lipid metabolism, energy and fatty acid metabolism were observed in the progression of atherosclerosis in apoE(-/-) mice. This study demonstrated that (1)H NMR-based metabonomics can provide biochemical information about the progression of atherogenesis and offer a non-invasive means to discover potential biomarkers for the onset and development of atherosclerosis.
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