A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.
A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.
复制标题
Gigantidas platifrons 中发现的 Toll 样受体及其在内共生甲烷氧化细菌免疫识别中的潜在作用
作者：
Li M;Chen H;Wang M;Zhong Z;Wang H;Zhou L;Zhang H;Li C
Symbiosis with chemosynthetic bacteria is an important ecological strategy for the deep-sea megafaunas including mollusks, tubeworms and crustacean to obtain nutrients in hydrothermal vents and cold seeps. How the megafaunas recognize symbionts and establish the symbiosis has attracted much attention. Bathymodiolinae mussels are endemic species in both hydrothermal vents and cold seeps while the immune recognition mechanism underlying the symbiosis is not well understood due to the nonculturable symbionts. In previous study, a lipopolysaccharide (LPS) pull-down assay was conducted in Gigantidas platifrons to screen the pattern recognition receptors potentially involved in the recognition of symbiotic methane-oxidizing bacteria (MOB). Consequently, a total of 208 proteins including GpTLR13 were identified. Here the molecular structure, expression pattern and immune function of GpTLR13 were further analyzed. It was found that GpTLR13 could bind intensively with the lipid A structure of LPS through surface plasmon resonance analysis. The expression alternations of GpTLR13 transcripts during a 28-day of symbiont-depletion assay were investigated by real-time qPCR. As a result, a robust decrease of GpTLR13 transcripts was observed accompanying with the loss of symbionts, implying its participation in symbiosis. In addition, GpTLR13 transcripts were found expressed exclusively in the bacteriocytes of gills of G. platifrons by in situ hybridization. It was therefore speculated that GpTLR13 may be involved in the immune recognition of symbiotic methane-oxidizing bacteria by specifically recognizing the lipid A structure of LPS. However, the interaction between GpTLR13 and symbiotic MOB was failed to be addressed due to the nonculturable symbionts. Nevertheless, the present result has provided with a promising candidate as well as a new approach for the identification of symbiont-related genes in Bathymodiolinae mussels.
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影响因子：
4.4
作者：
Detree, Camille;Haddad, Iman;Mary, Jean
通讯作者：
Mary, Jean
影响因子：
4.7
作者：
Barros, Ines;Divya, Baby;Bettencourt, Raul
通讯作者：
Bettencourt, Raul
影响因子：
5.1
作者：
Fontanez, Kristina M.;Cavanaugh, Colleen M.
通讯作者：
Cavanaugh, Colleen M.
DOI：
10.1016/j.cbpb.2017.02.002
发表时间：
2017-05-01
影响因子：
2.2
作者：
Detree, Camille;Lallier, Francois H.;Mary, Jean
通讯作者：
Mary, Jean
影响因子：
64.8
作者：
KENNICUTT, MC;BROOKS, JM;MCDONALD, TJ
通讯作者：
MCDONALD, TJ