Uromycobiome in infants and toddlers with and without urinary tract infections.
Uromycobiome in infants and toddlers with and without urinary tract infections.
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DOI：
10.1007/s00467-022-05844-3
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发表时间：
2023-07
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影响因子：
--
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中科院分区：
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--
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已经在儿童中描述了尿路细菌组的细菌成分，包括有和没有尿路感染（UTI）的儿童。然而，对儿科泌尿真菌生物群（尿液中的真菌群落）知之甚少。本研究的目的是描述儿童的泌尿真菌群，并确定泌尿真菌群是否在UTI儿童和非UTI儿童之间存在差异。这是一项横断面研究，对象是3岁以下的发热儿童，他们在急诊室就诊，并在临床护理中接受了导尿管尿样。我们获得了用于本研究的残余尿，并通过真菌ITS2区域的扩增和测序鉴定了尿肌生物组的组分。然后，我们比较了有和没有UTI的人之间的泌尿真菌组。我们在这项研究中纳入了374名儿童（UTI=50，无UTI =324）。从310名（83%）儿童的尿液样本中分离出真菌。与未患UTI的儿童相比，在UTI儿童中发现真菌的比例更高（96% vs 81%，p=0.01）。与没有UTI的儿童相比，UTI儿童的Shannon多样性指数更高（p=0.04）。虽然有和没有UTI的儿童之间的最丰富的类群有差异，有没有组之间的β多样性的显着差异。在大多数儿童的导尿管尿液样本中检测到真菌。虽然与没有UTI的儿童相比，UTI儿童尿液中真菌的比例较高，但这些组的组成没有差异。
The bacterial components of the urobiome have been described in children, both with and without urinary tract infections (UTI). However, less is known about the pediatric uromycobiome: the community of fungi in the urine. The objectives of this study were to describe the uromycobiome in children and determine whether the uromycobiome differs between children with and without UTI. This was a cross-sectional study of febrile children less than three years of age who presented to the Emergency Department and had a catheterized urine sample sent as part of clinical care. We obtained residual urine for use in this study and identified components of the uromyobiome through amplification and sequencing of the fungal ITS2 region. We then compared the uromycobiome between those with and without UTI. We included 374 children in this study (UTI=50, no UTI =324). Fungi were isolated from urine samples of 310 (83%) children. Fungi were identified in a higher proportion of children with UTI compared to those without UTI (96% vs 81%, p=0.01). Shannon diversity index was higher in children with a UTI compared to those without (p=0.04). Although there were differences in the most abundant taxa between children with and without UTI, there was no significant difference in beta diversity between groups. Fungi was detected in the majority of catheterized urine samples from children. While a higher proportion of children with UTI had fungi in their urine compared to children without UTI, there was no difference in the composition of these groups.
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影响因子：
3.7
作者：
Burton M;Krumbeck JA;Wu G;Tang S;Prem A;Gupta AK;Dawson TL Jr
通讯作者：
Dawson TL Jr
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.2
作者：
Bertini, Alessia;Zoppo, Marina;Tavanti, Arianna
通讯作者：
Tavanti, Arianna
影响因子：
3.4
作者：
Nickel, J. Curtis;Stephens, Alisa;Ehrlich, Garth D.
通讯作者：
Ehrlich, Garth D.
影响因子：
2
作者：
Storm, Douglas W.;Copp, Hillary L.;Wolfe, Alan J.
通讯作者：
Wolfe, Alan J.